Non-specific protein

Hi all, During nuclear protein purification I came across the step where I need to add 5M NaCl slowly with shaking until I adjust the molarity to 2M NaCl, how many mls do I need to add? Honestly I tried to tackle the problem in different ...

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Your protein is crucial for myoblast survival?

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But the buffer in the new ladder should be the same as the others. The ladder is basically combining 2ul of each of the 9 dye-conjugated proteins (~18ul total). The dye-conjugated protein that I loaded separately and combined in the ladder are from the same solution. In addition, I loaded ...

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Hi there, I have to do a cell wall extraction on bacteria soon and my protein of interest is cell wall anchored. My supervisor told me to do TCA precipitation (haven't done before) but I assume it's too isolate the proteins within the cell wall?? He told me ...

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Hi everyone! I'm trying to construct a protein ladder, which are markers used to indicate the molecular weight of proteins when running a SDS-PAGE. I have conjugated several different molecular weight proteins with dyes and ran them on a 12% ...

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