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Nif gene

nif gene

(Science: molecular biology) The complex of genes in nitrogen fixing bacteria that code for the proteins required for nitrogen fixation, particularly the nitrogenase.

present as an operon in klebsiella and carried on plasmid in rhizobium which inhabit the roots of leguminous plants.


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Problems with gene knockdown

We use RNAi and Lentiviral transgenesis in our lab to generate transgenic mice. We use luceferase assay to validate the shRNA sequences. The problem is the results don't seem to traslate well into in vitro systems. I analysed 8 sequences ...

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by zodiac1505
Tue Sep 08, 2009 11:31 am
 
Forum: Molecular Biology
Topic: Problems with gene knockdown
Replies: 0
Views: 27

Mitochondrial Gene Replacement

Researchers have figured out a potential way to prevent mitochondrial DNA related diseases by trading out healthy mitochondria for the mutated mitochondria. Apparently there are no side effects. Read more

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by jyaron
Sun Sep 06, 2009 4:01 am
 
Forum: Genetics
Topic: Mitochondrial Gene Replacement
Replies: 0
Views: 40

The gene P21 makes people live short or longer?

All genes are necessary and required.. that's the general rule of genomics. It's mutations in genes that affect the "good" or "bad" effects of the gene in particular. Considering the pathway the p21 gene ...

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by jyaron
Sat Sep 05, 2009 10:36 pm
 
Forum: Genetics
Topic: The gene P21 makes people live short or longer?
Replies: 1
Views: 48

Primer problem

... us unwanted banding in our gels and it turned out that our primers were fine. Maybe you could try ordering a primer for a different section of the gene? We usually order 2-3 different primers for any given gene we're testing for.. after all, it is pretty inexpensive.

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by jyaron
Sat Sep 05, 2009 10:29 pm
 
Forum: Molecular Biology
Topic: Primer problem
Replies: 2
Views: 20

Primer problem

Hi I designed a specific primers and blast it to amplify my gene. this primer amplifies a gDNA of the gene. I used dreamtaq kit and its protocol but unfortunately the expected product (2054 bp) was not there and what I got is several bands below and above ...

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by roniadam
Sat Sep 05, 2009 3:10 am
 
Forum: Molecular Biology
Topic: Primer problem
Replies: 2
Views: 20
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