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Nick translation



A tagging technique in molecular biology in which DNA Polymerase I is used to replace some of the nucleotides of a DNA sequence with their labeled analogues, creating a tagged DNA sequence which can be used as a probe in fluorescent in situ hybridization or blotting techniques.


Nick translation is the most commonly used method for labeling probes for in situ hybridization. It derived its name from the single-stranded nicks resulting from the DNA treated with DNAse, and the nick has "translated" some distance depending on the processivity of the polymerase.

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Re: Theories - Origin of Life

... cell. These have their own circular genome carry out transcription and translation and replicate independently of the host cell by binary division, ... the additional problem of energy requirements. In a review article by Nick Lane and William Martin published in Nature in Oct 2010 http://www.nature.com/nature/journal/v467/n7318/full/nature09486.html ...

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by scottie
Mon Jan 30, 2012 3:56 pm
Forum: Evolution
Topic: Theories - Origin of Life
Replies: 563
Views: 1127477

Reasons for Skepticism on the Endosymbiosis?

... In November 2010, I drew attention to a paper in Nature by Nick Lane and Bill Martin, who showed that the prokaryote-to-eukaryote transition ... independent from the nuclear DNA) in which transcription is coupled to translation, characteristic of bacterial DNA. There are also some other notable ...

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by SuperfluousAvatar
Thu Jan 12, 2012 7:43 pm
Forum: Cell Biology
Topic: Reasons for Skepticism on the Endosymbiosis?
Replies: 0
Views: 7603

About DNA & RNA

... 8.primase a. prohibits super-coiling of DNA molecule b. repairs the 'nick' left in DNA c. catalyze condensation bonding between amino acids d. ... 6)D 7)B 8)F multiple choice bold ones are my answers 1a)Accuracy in the translation of mRNA into the primary structure of a protein depends on specificity ...

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by wille2511
Wed Dec 05, 2007 2:10 am
Forum: Molecular Biology
Topic: About DNA & RNA
Replies: 2
Views: 2417

One question about Fluorescence in situ Hybridization(FISH)

... necessary? and why? I want to use BAC clone as probe (labelled with DIG-Nick Translation MIX, from Roche) and detected with Fluorescent antibody enhancer set for DIG Detection ...

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by zeyuanzhu
Mon Nov 05, 2007 1:10 am
Forum: Molecular Biology
Topic: One question about Fluorescence in situ Hybridization(FISH)
Replies: 0
Views: 1498

The Idea

... E.Coli PolI but its Klenow fragment. A Klenow fragment lacks 'nick-translation' activity. Therefore the RNA primers are not degraded. Hence at the end of the ...

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by cytochromeP
Tue Aug 23, 2005 7:10 am
Forum: Molecular Biology
Topic: Centrifugation query
Replies: 13
Views: 7661

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