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Neutral stain

neutral stain

(Science: technique) A compound of an acid stain and a basic stain, such as the eosinate of methylene blue, in which the anion and cation each contains a chromophore group.

Synonym: salt dye.

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Re: HELP! Cannot decide which Gram+, rod-shaped organism this is

Think you'd better try that Gram stain again. Among the species you offered is an anaerobic bacterium, one that grows poorly if at all on media neutral in pH, more than one that's nonmotile and a number that produce obvious spores.

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by JorgeLobo
Sun Apr 01, 2012 12:29 am
Forum: Microbiology
Topic: HELP! Cannot decide which Gram+, rod-shaped organism this is
Replies: 3
Views: 2481

Re: How to stain vacuoles of fungi

It's a bit difficult to stain nothing so how about staining the tissue around them. I presume you're using a light microscope how about trying vital stains of either neutral red or trypan blue

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by ches555
Fri Jul 02, 2010 10:14 am
Forum: Microbiology
Topic: How to stain vacuoles of fungi
Replies: 1
Views: 1191

M Biology

... Raised, smooth, glistening Cell morphology Rod, Bacillus (single) Gram stain Pink, Gram - Osmotic sensitivity Up to 20% NaCl Temperature tolerance 4˚ – 37˚ - Mesophile Acidity/alkalinity Prefers Alkaline/neutral Growth on glucose No Growth Growth on amino acids (a.a.) Growth Growth ...

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by ottotrac
Wed Apr 22, 2009 4:43 pm
Forum: Microbiology
Topic: M Biology
Replies: 0
Views: 3697

Spermatozoa staining

I need references to stain spermatozoa in order to investigate their viability/vitality. I read that double-staining (trypan blue/congo red + Giemsa) is good to differ dead/alive and also the presence of acrosome. Triple-staining (eosin/nigrosin+Bismarck ...

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by DoctorStein
Sun Jun 29, 2008 11:14 am
Forum: Cell Biology
Topic: Spermatozoa staining
Replies: 0
Views: 2340

The Fiber Disease

... to sensing cell of plant and insect. Try diluted copper sulphate in a neutral base( baby shampoo) Keep wet for a while and shower. Repeat. ---------------------------------------------------- ... Observe how lesions stain, dry out and cure within days. Your infestation with sensing protein ...

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by tamtam
Sun Oct 30, 2005 9:58 pm
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 6263511

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