Dictionary » N » Negative stain

Negative stain

negative stain

(Science: technique) stain forming an opaque or coloured background against which the object to be demonstrated appears as a translucent or colourless area; in electron microscopy, an electron opaque material, such as phosphotungstic acid or sodium phosphotungstate, is used to give detail as to surface structure.


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Gram Positive Cocci

... far: Colony morphology: Yellow, round, slightly concave, smooth. Gram stain: Gram positive, appears to be cocci, but not perfectly round and tend ... mean that it ferments sucrose instead of lactose?) Nitrate Reduction: Negative (does not break down NO) Catalase: Positive Based on the first dichotomous ...

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by lucrezia03
Wed Oct 17, 2012 11:58 pm
 
Forum: Microbiology
Topic: Gram Positive Cocci
Replies: 2
Views: 777

gram negative cocci. please help

You said you did an ACID FAST test to show you if it was GRAM NEGATIVE? Did you do a Gram stain at all? An acid-fast test doesn't tell you if it's Gram negative or Gram positive. Rather, ...

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by keetner
Mon Oct 08, 2012 12:23 am
 
Forum: Microbiology
Topic: gram negative cocci. please help
Replies: 7
Views: 4318

Unknown Organism

... help identifying my unknown by Monday. Here are the results. 1. Gram Stain Positive (Clustered Bacilli) 2. Capsule Negative 3. Endospore Negative* 4. Phenol Red Broth Fermentation with Acid and Gas end products ...

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by CheriPeace
Fri Jun 29, 2012 9:08 pm
 
Forum: Microbiology
Topic: Unknown Organism
Replies: 5
Views: 2366

Re: Microbiology help in identifying Gram + rod for my unknown

... Cell Shape: Bacilli Arrangement: Diplibacilli Size: 2mm X .5mm Gram Stain: Negative Motility: Yes Glucose Fermanation: yes acid/gas Lactose " ": yes acid/gas ...

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by sunshineshoes
Tue Jun 26, 2012 1:02 pm
 
Forum: Microbiology
Topic: Microbiology help in identifying Gram + rod for my unknown
Replies: 12
Views: 4166

Proliferation assay

How does the single peak relate to your negative control? Do all your cells have full or lowered CFSE intensity? Does the ... divisions -> one peak. You could try to control this by comparing your unstained cells, fresh CFSE stain and one sample from, say, day 4 or 5. If the day ...

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by biohazard
Wed Jun 13, 2012 7:17 am
 
Forum: Cell Biology
Topic: Proliferation assay
Replies: 7
Views: 7915
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