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Necrotic

necrotic

(Science: medicine) affected with necrosis; as, necrotic tissue; characterised by, or producing, necrosis; as, a necrotic process.


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Evolution: which came first, classical apoptosis or necrosis

As far as I know, necrosis, by definition, is cell death due to external factors that end cell's life prematurely. Thus, there is no necrotic pathway - necrosis is not a physiological phenomenom. Furthermore, as long as there has been a living cell, there must have been necorisis. So I really ...

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by biohazard
Mon Oct 12, 2009 6:39 am
 
Forum: Molecular Biology
Topic: Evolution: which came first, classical apoptosis or necrosis
Replies: 2
Views: 1081

Evolution: which came first, classical apoptosis or necrosis

... evolved a better system => apoptosis. At the same time, most cells try to go for apoptosis and if there's not enough energy they go the necrotic-type way, so injury apoptosis could have evolved as a backbone, a safety net for apoptosis- just in case there isn't enough energy. PLEASE ...

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by tobio1
Mon Oct 12, 2009 4:48 am
 
Forum: Molecular Biology
Topic: Evolution: which came first, classical apoptosis or necrosis
Replies: 2
Views: 1081

Re: Lymphocyte Overnight Culture parameters

... worked best. Cell washing medium (for centrifuge): RPMI + 2% FCS Cell culture medium : RPMI + 15% FCS 5% CO2 @ 37C. After 24h incubation, necrotic cells were around 0.6% and late apoptotic cells 4.5%

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by watsond
Wed Dec 03, 2008 11:15 am
 
Forum: Molecular Biology
Topic: Lymphocyte Overnight Culture parameters
Replies: 5
Views: 8990

troponin

Troponin is a serum cardiac marker that is released into the blood in large quantities from the necrotic heart muscle after a heart attack. It is a very good indicator because it remains elevated two weeks after the initial attack. Also, as a myocardial muscle protein, ...

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by bionewbie
Thu Apr 12, 2007 1:14 am
 
Forum: Human Biology
Topic: troponin
Replies: 2
Views: 3183

Adipocytes preparation for FACS (cells from tissue)

... of adipocytes for FACS analysis. Annexin V and propidium iodide can be used in FACS to discriminate quantitatively between apoptotic cells and necrotic cells. Annexin and PI will not penetrate into intact cells, while cells that will be stained by Annexin only indicate apoptotic cells, and ...

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by lior
Mon Jan 29, 2007 7:51 am
 
Forum: Cell Biology
Topic: Adipocytes preparation for FACS (cells from tissue)
Replies: 2
Views: 3166
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