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Native protein

Definition

noun, plural: native proteins

The protein inside the cell that is in its native or natural state and unaltered by denaturing agent, such as heat, chemical, enzyme action, or the exigencies of extraction.


Supplement

A native protein is in its functional form, particularly in a simple, unbranched chain of amino acids while assuming a specific 3D shape.


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Over expressing proteins in mammalian cells

if it's mammalian protein than you have almost native conditions However, it's not so easy to handle.

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by JackBean
Wed Feb 20, 2013 1:32 pm
 
Forum: Genetics
Topic: Over expressing proteins in mammalian cells
Replies: 2
Views: 1079

Interpretation & Troubleshooting on SDS/Native PAGE

The Native PAGE looks really bad, it probably didn't really enter the gel. What percentage was ... How does your sample look now on gel exclusion chromatography? What was source of your protein? I guess it wasn't native enzyme, because then you could hardly have only three bands ...

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by JackBean
Sun Dec 16, 2012 10:17 pm
 
Forum: Molecular Biology
Topic: Interpretation & Troubleshooting on SDS/Native PAGE
Replies: 1
Views: 1826

Interpretation & Troubleshooting on SDS/Native PAGE

... running both DEAE and gel filtration purification, I did both SDS and Native PAGE to verify my results. For SDS PAGE, there were 3 faint bands ... loading well, I suspect it wasn't just my enzyme but it probably is a protein aggregate. How do I verify this i.e. is it only one protein or protein ...

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by schroddycat
Sat Dec 15, 2012 4:12 pm
 
Forum: Molecular Biology
Topic: Interpretation & Troubleshooting on SDS/Native PAGE
Replies: 1
Views: 1826

Radio-phosphate binding assay

I'm having trouble with a test. I want to know how many proteins in E.coli (ER2566) bind to phosphate (H3PO4). This is my test: 1) Extract ... in 10ml 10mM Tris-Cl buffer ph 7.0. 2) Pass 500ul protein through a native PAGE (I have combs this big). 3) Western blotting: transfer to an activated ...

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by vader1941
Tue Jul 10, 2012 3:32 pm
 
Forum: Molecular Biology
Topic: Radio-phosphate binding assay
Replies: 0
Views: 1524

Re:

... physical link between DNA sequences or physical link between encoded proteins and not physical link between DNA and protein, i.e. the DNA is connected ... and the DNA sequence encoding it" Perhaps its because I am not a native speaker... But I dont find it clearly stated, to me it does sound ...

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by poloke
Sat Jun 02, 2012 3:13 pm
 
Forum: Microbiology
Topic: Question about phage library
Replies: 3
Views: 2603
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