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Native conformation

native conformation

(Science: chemistry) The conformation in which a molecule is biologically active.

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Re: SDS-PAGE help please

... for and breaking disulfide bonds that hold the protein together in its native conformation (highly folded). DTT and B-mercaptoethanol are typical reducing agents used for ...

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by MJD
Thu Oct 01, 2009 2:58 am
Forum: Bioinformatics
Topic: SDS-PAGE help please
Replies: 6
Views: 14477

at sona regarding anfindsens expt

... was also required, to reduce the 4 disulfide linkages of the native conformation The experiment showed that if the disulfide bonds were allowed to form in the ...

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by hisunny4u
Mon Oct 01, 2007 11:34 am
Forum: Genetics
Topic: Anfinsens Experiment
Replies: 5
Views: 12183

fusion protein

... a structure that was the same in both the fusion protein and the native structure. The question was, for a small peptide--something considerably ... stucture on such a peptide, or would the peptide retain its native conformation, and the answer, at least for this peptide, was that it retained ...

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by blcr11
Tue Apr 10, 2007 4:41 pm
Forum: Molecular Biology
Topic: fusion protein
Replies: 8
Views: 4539

Question about DNA conformation

Does the conformation of both native and denatured DNA show any difference? I'm looking for double-stranded DNA which is in helix form in solution. ...

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by ahkian82
Mon Jan 22, 2007 6:40 am
Forum: Molecular Biology
Topic: Question about DNA conformation
Replies: 4
Views: 2723

Need Help with some Chemical Components For Those Smart Peop

... bond reducers etc..) when the other one helps keep proteins in their native conformation. As for your phosphorylation buffer it probably contains the reagents necesaary ...

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by canalon
Wed Apr 12, 2006 5:34 pm
Forum: Molecular Biology
Topic: Need Help with some Chemical Components For Those Smart Peop
Replies: 3
Views: 1868

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