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Nadp -dependent serine dehydrogenase

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MOLECULAR CLONING

Not to mention, that synthesized oligos have 5'-OH and 3'-phosphate ends... DNA ligase removes 3'-phosphates and replaces it with -OH. Thus, synthesized oligos without polynucleotide kinase treatment will participate in the ligation reaction with both their ends having -OH groups. The 3'-OH end of t...

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by kk
Fri Jul 19, 2013 12:50 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 7036

MOLECULAR CLONING

Thanks for the reply. I understand that dephosphorylation would prevents self-ligation. My problem was that whether annealed double stranded oligos need to be modified in any way before the ligation reaction. And yes, they need a 5'-phosphate added, by polynucleotide kinase, so the ligase can actual...

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by kk
Wed Jul 10, 2013 2:58 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 7036

Re: Codons are triplets, but what's a Singlet, Doublet, etc?

Ah ha! Thinking it terms of words/letters sent Google Scholar straight for something in a 1982 paper that I did not know about, where in its Abstract it theorizes a two letter codon: THE POSSIBLE ROLE OF ASSIGNMENT CATALYSTS IN THE ORIGIN OF THE GENETIC CODE VAHE BEDIAN * Department of Biophysical S...

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by GaryGaulin
Thu Oct 25, 2012 4:28 am
 
Forum: Bioinformatics
Topic: Codons are triplets, but what's a Singlet, Doublet, etc?
Replies: 4
Views: 14451

Re: Campbell and Reece 8th edition

thank you for that Pg 314 “The unwound sections of parental DNA strands are now available to serve as templates for the synthesis of a polynucleotide; they can only add nucleotides to the end of an already existing chain that is produced during DNA synthesis is actually a short stretch of RNA, not D...

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by Stephen1993
Wed May 11, 2011 12:32 pm
 
Forum: Cell Biology
Topic: Campbell and Reece 8th edition
Replies: 36
Views: 30344

I need some help on these questions

These are on my practice test and these are the only ones stumping me, thanks in advance! Biological species consist of? Populations, domains, families,phyla, or genera Which protozoan group consists solely of parisitc forms? Amoebas, ciliates, slime molds, apicomplexans, flagellates If adenine make...

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by 2001ChevyZ71
Sun Nov 28, 2010 3:20 am
 
Forum: General Discussion
Topic: I need some help on these questions
Replies: 4
Views: 7428
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