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Myoblasts

myoblast

(Science: pathology) cell that by fusion with other myoblasts gives rise to myotubes that eventually develop into skeletal muscle fibres. The term is sometimes used for all the cells recognisable as immediate precursors of skeletal muscle fibres. Alternatively, the term is reserved for those post mitotic cells capable of fusion, others being referred to as presumptive myoblasts.


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Xenotransplantation - graft rejection detection??

Hi everyone, I am working on an in vivo experiment in rats where I implanted (subcutaneously) genetically modified C2C12 murine myoblasts that deliver Epo enveloped in a delivery system we are working on in our lab. Some of the experimental groups were transiently immunosupressed and others ...

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by Ainhoa M
Thu Feb 21, 2008 11:23 am
 
Forum: Cell Biology
Topic: Xenotransplantation - graft rejection detection??
Replies: 1
Views: 589

Unhealthy C2C12

I am worried about my C2C12 myoblasts at the moment - they were looking "spindley" last week so I got rid of them, made up fresh media and fresh PBS and broke out a fresh batch of cells. However they still looked the same today ...

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by maddensd
Thu Apr 20, 2006 4:28 pm
 
Forum: Cell Biology
Topic: Unhealthy C2C12
Replies: 4
Views: 848

C2C12 myoblasts - attachment problems!!!

Good afternoon, Can anyone solve me this problem please? C2C12 myoblasts, always grown attached to culture flasks...Suddenly one week ago more or less lots of them are free-floating forming aggregates most of the time...although lots are also growing attached ...

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by Ainhoa M
Thu Mar 30, 2006 4:01 pm
 
Forum: Cell Biology
Topic: C2C12 myoblasts - attachment problems!!!
Replies: 1
Views: 637

Angiogenesis-related markers

Hi all! Could anyone tell me which markers are most suitable to visualize neo-vascularization in vitro/ in vivo? (I work with C2C12 myoblasts) Some examples I already know are: CD31, desmin and myogein immunostaining, Von Willebrand factor, H/E I have found these ones in the literature, ...

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by Ainhoa M
Wed Mar 29, 2006 11:00 am
 
Forum: Cell Biology
Topic: Angiogenesis-related markers
Replies: 0
Views: 513

Desferal - Intramuscular Administrations??

... Under hypoxic conditions, this substance activates HIF-1 and so EPO is produced. The thing is that we have to inject genetically engineered C2C12 myoblasts treated with desferal into mice (I've read this has to be done intramuscularly) but I can't find how to do so correctly. I read that it can ...

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by Ainhoa M
Tue Jan 31, 2006 9:14 am
 
Forum: Cell Biology
Topic: Desferal - Intramuscular Administrations??
Replies: 0
Views: 535
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