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Myoblasts

myoblast

(Science: pathology) cell that by fusion with other myoblasts gives rise to myotubes that eventually develop into skeletal muscle fibres. The term is sometimes used for all the cells recognisable as immediate precursors of skeletal muscle fibres. Alternatively, the term is reserved for those post mitotic cells capable of fusion, others being referred to as presumptive myoblasts.


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Conditional knockout mouse

... inducible Cre line, CAGGCre-ERTM, I get mice with with heterozygous Cre and floxed gene of interest. The experimental plan was to isolate myoblasts from these mice and induce Cre in cell culture by addition of Tamoxifen. To my surprise, the cells isolated from these mice die with in few ...

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by aji01
Wed Feb 20, 2013 11:25 am
 
Forum: Genetics
Topic: Conditional knockout mouse
Replies: 1
Views: 1569

Problems with C2C12 resuscitation

... This is my first post here, and I hope you could help me. I'm restarting a lab, and I'm experiencing trouble thawing my C2C12 cells (mouse myoblasts). I thawed my ampule rapidly in a 37°C waterbath, and wipe my ampule with ethanol. I then pipette the content of the ampule in 5 ml of prewarmed ...

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by CecileL
Wed Jun 23, 2010 7:50 am
 
Forum: Cell Biology
Topic: Problems with C2C12 resuscitation
Replies: 1
Views: 1983

Stem Cell Injection

I think, they should replace damaged myoblasts, shouldn't they?

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by JackBean
Tue Mar 09, 2010 7:55 am
 
Forum: General Discussion
Topic: Stem Cell Injection
Replies: 8
Views: 4048

Xenotransplantation - graft rejection detection??

Hi everyone, I am working on an in vivo experiment in rats where I implanted (subcutaneously) genetically modified C2C12 murine myoblasts that deliver Epo enveloped in a delivery system we are working on in our lab. Some of the experimental groups were transiently immunosupressed and others ...

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by Ainhoa M
Thu Feb 21, 2008 11:23 am
 
Forum: Cell Biology
Topic: Xenotransplantation - graft rejection detection??
Replies: 1
Views: 3519

Unhealthy C2C12

I am worried about my C2C12 myoblasts at the moment - they were looking "spindley" last week so I got rid of them, made up fresh media and fresh PBS and broke out a fresh batch of cells. However they still looked the same today ...

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by maddensd
Thu Apr 20, 2006 4:28 pm
 
Forum: Cell Biology
Topic: Unhealthy C2C12
Replies: 4
Views: 1792
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