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Mrna

Definition

noun

Abbreviated form for messenger ribonucleic acid, the type of RNA that codes for the chemical blueprint for a protein (during protein synthesis).


Supplement

In prokaryotes, mRNA is derived from a large primary transcript from a DNA sequence. The mRNAs of prokaryotes are usually very short lived (from seconds to more than an hour) and protein synthesis starts even while the mRNA is still being synthesized. The resulting mRNA is essentially mature upon transcription and requires no extensive processing.

In eukaryotes, the mRNA is produced in the nucleus (during transcription). This mRNA needs to be processed extensively to become mature. This extensive processing includes the addition of a 5' cap at the 5' end and a sequence of adenylate groups at the 3' end, the poly a tail, as well as the removal of any introns and the splicing together of exons. When the pre-mRNA has been completely processed, it is now called a mature mRNA, which will then be transported for translation into the cytoplasm through the nuclear pore. The eukaryotic mRNA has relatively longer lifespan, e.g. mammalian mRNA can live from several minutes to days.


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Results from our forum


mRNA vs. cell reporter assay

... interpretation of the GTP signal. if you are looking for downregulation of transcription, it takes a while for preexisting GPR to degrade but the mRNA signal is likely to disappear fairly rapidly once transcription stops. For upregulation, mRNA will reach steady-state fairly rapidly but there ...

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by jonmoulton
Mon Sep 08, 2014 5:27 pm
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 381

Re: Totipotent Stem Cell From Molecular Components

... for a particular protein in my stem cell at least, the amount of that particular protein is the average of the gene expression in # of strands of mRNA * 100. I am getting the protein this way. I take the results of PCR and put it in a test tube with RNA polymerases, Ribosomes, and other proteins ...

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by caters
Fri Aug 01, 2014 1:42 am
 
Forum: Cell Biology
Topic: Totipotent Stem Cell From Molecular Components
Replies: 2
Views: 2759

mRNA vs. cell reporter assay

... upon activation of a transcription factor. In theory, I can study transcription factor activation via the GFP signal, or by measuring the mRNA of the transcription factor. I wonder what are the pros and cons of each. Can anyone help me figure this out? Thanks, John

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by aguy
Mon Jul 07, 2014 2:50 am
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 381

Totipotent Stem Cell From Molecular Components

... type should I do this: Go to BioGPS Look up the proteins Make sure I have human selected(since I am making a human stem cell) Average the mRNA expression to get stem cell mRNA expression Multiply by 100 to get the amount of protein Q4 I have heard of amplicons used in PCR. Do I really ...

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by caters
Sun Feb 02, 2014 10:58 pm
 
Forum: Cell Biology
Topic: Totipotent Stem Cell From Molecular Components
Replies: 2
Views: 2759

Re: qPCR vs Northern Blot

... you're rigth first - in Northern blot I had silencing, in qPCR i didn't. As far as your question is concerned - my NB probe covers whole length of mRNA - all exons (it's a short one ~2 kbp total). In case of my PCR primers - FORWARD primer binds in one exon in the middle of mRNA, and REVERSE binds ...

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by Nevthonien
Tue Nov 12, 2013 2:47 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 2578
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