Dictionary » M » Mrna

Mrna

Definition

noun

Abbreviated form for messenger ribonucleic acid, the type of RNA that codes for the chemical blueprint for a protein (during protein synthesis).


Supplement

In prokaryotes, mRNA is derived from a large primary transcript from a DNA sequence. The mRNAs of prokaryotes are usually very short lived (from seconds to more than an hour) and protein synthesis starts even while the mRNA is still being synthesized. The resulting mRNA is essentially mature upon transcription and requires no extensive processing.

In eukaryotes, the mRNA is produced in the nucleus (during transcription). This mRNA needs to be processed extensively to become mature. This extensive processing includes the addition of a 5' cap at the 5' end and a sequence of adenylate groups at the 3' end, the poly a tail, as well as the removal of any introns and the splicing together of exons. When the pre-mRNA has been completely processed, it is now called a mature mRNA, which will then be transported for translation into the cytoplasm through the nuclear pore. The eukaryotic mRNA has relatively longer lifespan, e.g. mammalian mRNA can live from several minutes to days.


Related terms:


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


miRNA-mediated competing endogenous RNAs (ceRNAs) networks

ceRNABase have constructed pan-cancer ceRNA networks from tumor miRNA and mRNA expression profiles (TCGA 14 cancer types with >6000 tumor and normal samples). For example: known PTEN-ZEB2 ceRNA interactions can be supported in 13 cancer types .

See entire post
by rnaworld2110
Mon Mar 03, 2014 11:42 am
 
Forum: Cell Biology
Topic: miRNA-mediated competing endogenous RNAs (ceRNAs) networks
Replies: 2
Views: 1165

Pan-Cancer pattern of lncRNAs, miRNAs, mRNAs

... and miRNAs across diverse cancer types. Pan-Cancer miRNA and lncRNA resources: (1) panCancerMine : explore Pan-Cancer pattern lncRNA, miRNA, mRNA and their regulatory networks (ceRNA, coexpression) by mining expression profiles of miRNAs, lncRNAs and mRNAs across 14 cancer types (>6000 ...

See entire post
by rnaworld2110
Thu Feb 06, 2014 11:56 am
 
Forum: Molecular Biology
Topic: Pan-Cancer pattern of lncRNAs, miRNAs, mRNAs
Replies: 0
Views: 238

Totipotent Stem Cell From Molecular Components

... type should I do this: Go to BioGPS Look up the proteins Make sure I have human selected(since I am making a human stem cell) Average the mRNA expression to get stem cell mRNA expression Multiply by 100 to get the amount of protein Q4 I have heard of amplicons used in PCR. Do I really ...

See entire post
by caters
Sun Feb 02, 2014 10:58 pm
 
Forum: Cell Biology
Topic: Totipotent Stem Cell From Molecular Components
Replies: 0
Views: 650

Re: qPCR vs Northern Blot

... you're rigth first - in Northern blot I had silencing, in qPCR i didn't. As far as your question is concerned - my NB probe covers whole length of mRNA - all exons (it's a short one ~2 kbp total). In case of my PCR primers - FORWARD primer binds in one exon in the middle of mRNA, and REVERSE binds ...

See entire post
by Nevthonien
Tue Nov 12, 2013 2:47 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 1320

Re: qPCR vs Northern Blot

... primers? If not, it is conceivable that a change in splicing in the presence of the shRNA causes your Northern target to be spliced out of the pre-mRNA while the binding sites of the PCR primers remain. This would match your observations of a decrease in the Northern signal but maintenance of the ...

See entire post
by jonmoulton
Thu Nov 07, 2013 10:12 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 1320
View all matching forum results

This page was last modified 22:43, 21 September 2009. This page has been accessed 79,656 times. 
What links here | Related changes | Permanent link