Motif

... assay and made virus with them. The virus / plasmid has an antibiotic resistance gene which is expressed under the same promoter control ( mir30 motif). shRNAs were tested against both rat and mouse cDNAs in luciferase assay. The infected cells (in both rat and mouse cell lines) were selected ...

See entire post

I dont have enough experience to give lab advices..but you could use hydropathy plot for determing hydrophilic and hydrophobic parts of protein thus predicting its conformation

See entire post

... GenBank. Conserved regions were identified by using software Clustal X/W. Than primer A was designed based on the conserved amino acid sequence motif DD(T/I)(I/Y/F)D(A/V)Y(A/G). The sequence of sense primer A was 5’-GA(C/T) GA(C/T) III T(T/A)(T/C) GA(C/T) GCI (C/T)A(C/T) GG-3’. My question is ...

See entire post

... yeast gene using seceral data sources. I know that gene can be clustered using gene expression data and using other alternatives. I klnow that motif frequencies is also a nother solution. I have read in a paper that using frequency measure of motifs we can cluster genes. Can anyone tell me ...

See entire post

... reasons why knowing whether a SNP is in a conserved region could be useful. First, conserved regions are likely to encode a useful functional motif of a protein. Evolutionary theory suggests that conserved regions vary less because their sequence is selected for by natural selection. Think ...

See entire post