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Monolayers

monolayers

1. Films, one molecule thick, formed on water by certain substances, such as proteins and fatty acids, characterised by molecules containing some atom groupings that are soluble in water and other atom groupings that are insoluble in water.

2. A confluent sheet of cells, one cell deep, growing on a surface in a cell culture.


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Protein isolation

Hi, I am trying to quantify HIF 1 alpha protein level. Our scaffold is not a polystyrene plate, and the cells are not grown as 2D monolayers (I am looking at cell aggregates to be specific). Our original plan is to isolate the protein using trizol extraction reagent, then run an ELISA on ...

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by roth317
Tue Feb 12, 2013 9:25 pm
 
Forum: Molecular Biology
Topic: Protein isolation
Replies: 2
Views: 2093

The Fiber Disease

I do not know how DOE got into this, but, here they are: The caco-2 cell monolayers. http://www.osti.gov/energycitations/product.biblio.jsp?osti_id=5239861 skytroll

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by Skytroll
Sat Nov 04, 2006 4:18 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5569828


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