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Monocytes

Monocytes

Monocytes are a type of white blood cell and are part of the innate immune system of vertebrates including all mammals (including humans), birds, reptiles, and fish. Monocytes play multiple roles in immune function. Such roles include: (1) replenish resident macrophages and dendritic cells under normal states, and (2) in response to inflammation signals, monocytes can move quickly (approx. 8-12 hours) to sites of infection in the tissues and divide/differentiate into macrophages and dendritic cells to elicit an immune response. Half of them are stored in the spleen.[1] Monocytes are usually identified in stained smears by their large kidney shaped or notched nucleus.Monocytes are precursors to macrophages. Monocytes are produced by the bone marrow from hematopoietic stem cell precursors called monoblasts. [[Image:Monocyte.png --Nihit 04:10, 2 August 2012 (CDT)]]


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peripheral mononuclear cells handling

... and incubated my cells for some 5 to 15 mins in +4C with moderate success (some of the cells tend to die, probably because of the scraper). The monocytes require an hour or two in +37C in culture medium to attach to the plate surface, and thus after 24h they should be firmly stuck and you can ...

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by biohazard
Mon Nov 05, 2012 1:11 pm
 
Forum: Cell Biology
Topic: peripheral mononuclear cells handling
Replies: 3
Views: 2026

Re: peripheral mononuclear cells handling

Indeed want to study monocytes. I think I'm going to use PBS/edta and to scrap them with the tips of the pipette. Do you think it's a good idea?

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by claire84
Fri Nov 02, 2012 2:56 pm
 
Forum: Cell Biology
Topic: peripheral mononuclear cells handling
Replies: 3
Views: 2026

peripheral mononuclear cells handling

Depends on what subset of PBMCs you want to study. In general, blood cells are suspension cells and do not require trypsination, but monocytes are an exception: they adhere to certain types of plastics (which includes most cell culture plates) and must be removed with PBS/EDTA & cell ...

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by biohazard
Fri Nov 02, 2012 8:46 am
 
Forum: Cell Biology
Topic: peripheral mononuclear cells handling
Replies: 3
Views: 2026

Learning tissue culture, specifically moDC generation

Monocyte-derived DCs should be easy enough to culture even with limited experience. Basically you need cell-culture plates that allow monocytes to stick onto its surface (they attach readily to most "hard" plastic surfaces), serum-free culture medium (such as AIM-V), growth factors ...

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by biohazard
Fri Feb 03, 2012 2:09 pm
 
Forum: Cell Biology
Topic: Learning tissue culture, specifically moDC generation
Replies: 2
Views: 2213

Learning tissue culture, specifically moDC generation

... point where we need to start working with DCs instead of a model cell line. I know there aren't any immortal DC lines, so most people just induce monocytes. How feasible would it be to generate moDCs with limited tissue culture experience? Is it considered a very advanced technique? Our lab doesn't ...

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by chemicalbio
Thu Feb 02, 2012 7:22 pm
 
Forum: Cell Biology
Topic: Learning tissue culture, specifically moDC generation
Replies: 2
Views: 2213
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