Moles

... coefficient are typically M^-1 cm^-1; in that case, a 1cm cell path length cancels out the cm^-1 unit and you'll end up with a concentration in moles/liter. Watch the units on your extinction coefficient, sometimes they are different (you could, for instance, have an extinction coefficient calibrated ...

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Yes, n is amount of substance http://en.wikipedia.org/wiki/Amount_of_substance in moles if you have 0.5 mol in 0.1 l, than the concentration is 0.5 mol/0.1 l = 0.05 mol/l

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... of the Fluorescent compound against their RFU). 1) My issue here or what make it difficult for me - is to convert the those mM into grams, moles, mmoles etc - what molecular weight i will be referring to? is it substrate (which i doubt it - wrong), or the molecualr weight of the standard ...

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Dear Jack, Thanks for your reply. But can you give details how to convert concentration for instance in M, mM, uM into moles, mmoles, nmoles as i am kind of "stupid" sometime with the conversion between concentration and other formula. Please detailed example if you like? ...

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... of a DNA bp. Therefore, it's 660g/mole = 1 bp (so it's actually 660 (g/mole)/bp - that is why the answer is in g/mole). Now 1 mole of dsDNA = 2 moles of ssDNA since each molecule becomes 2 molecules when dissociated. And that is where 2 comes from.

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