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Molecular mass

molecular mass

The molecular mass of a substance (less accurately called molecular weight and abbreviated as MW) is the mass of one molecule of that substance, relative to the unified atomic mass unit u (equal to 1/12 the mass of one atom of carbon-12).


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Re: Cell and Molecular Revision Question Help!!!

This is what I have.. Consider the Vector (Mass of Vector): pBR328 (4.9 x 103) x 660 = 2.94x106 Daltons Given: Moles = Mass/MW Moles = 50 x 10-9g / 2.94x106 Moles = 17 x10-15 Each vector has two ends therefore x2 = x2 Therefore there are 34 x10-15 ...

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by Didact1
Mon Sep 30, 2013 10:02 pm
 
Forum: Molecular Biology
Topic: Cell and Molecular Revision Question Help!!!
Replies: 4
Views: 2667

Western Blot

... is to separate it according to how big it is, otherwise known as its molecular weight. When extracts are prepared from a cell, detergents can ... be separated by an electric current strictly according to their overall mass. (1)

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by MarkHolland
Sat Mar 30, 2013 3:22 pm
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 3274

Re: Calculating the molecular concentration of DNA

... ratio of dsDNA:ssDNA is 1:2 -- and like any ol' stoich problem, you would have to times by two in this case. But if we were STRICTLY talking about MASS and not concentration, then my logic in thinking you would divide by two would not be wrong, would it? Yes...? Hopefully?! I'm not sure if I'm ...

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by keetner
Sun Oct 07, 2012 11:11 pm
 
Forum: Molecular Biology
Topic: Calculating the molecular concentration of DNA
Replies: 6
Views: 5074

Re:

... as to why you would multiply by two (my second question). If 660 is the mass of the base PAIRS, I assume we are including the mass of both strands. ... Thanks again. Because 660 is NOT the mass of the bp. It's an average Molecular Weight of a DNA bp. Therefore, it's 660g/mole = 1 bp (so it's actually ...

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by Cat
Sun Oct 07, 2012 9:32 pm
 
Forum: Molecular Biology
Topic: Calculating the molecular concentration of DNA
Replies: 6
Views: 5074

Calculating the molecular concentration of DNA

... in double stranded DNA is 660, and that a single strand base is 330. - If this is the case, wouldn't each base pair technically have different masses? A-T vs C-G? 2) Why do we multiply by two? The question says "find molar concentration of single strands of DNA..." -If 660 is the ...

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by keetner
Sun Oct 07, 2012 7:49 pm
 
Forum: Molecular Biology
Topic: Calculating the molecular concentration of DNA
Replies: 6
Views: 5074
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