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Molarity

molarity

moles per liter of solution (mol/L).

Compare: molality.

Abbreviation: M


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Nuclear protein purification

Hi all, During nuclear protein purification I came across the step where I need to add 5M NaCl slowly with shaking until I adjust the molarity to 2M NaCl, how many mls do I need to add? Honestly I tried to tackle the problem in different ways but I couldnt, any help would be really appreciated. ...

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by bravebeaker
Mon Mar 18, 2013 7:49 am
 
Forum: Molecular Biology
Topic: Nuclear protein purification
Replies: 4
Views: 1740

Re: beers law concentration calculation

Ok I believe the reason I am confused is because in chemistry when we say "concentration" we usually mean molarity, or moles per unit volume. Here you are talking about concentration in terms of mass per volume, which I thought was density? Is it different for proteins ...

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by protchem12
Thu Jan 12, 2012 10:23 pm
 
Forum: Molecular Biology
Topic: beers law concentration calculation
Replies: 8
Views: 6273

Problem with transgenic mice

... to test all the variables (taq, dNTP's especially, to ensure the PCR worked) - (usually someone can offer you actin primers and template at known molarity). 1. Mg concentration is key - alter this, use the temperature that gave you the best result. 2. You are using cDNA - does this mean a crude ...

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by merv
Sun Oct 02, 2011 9:32 pm
 
Forum: Genetics
Topic: Problem with transgenic mice
Replies: 2
Views: 7198

PLease Please I need help with my PCR! Greatly appreciated!

... to test all the variables (taq, dNTP's especially, to ensure the PCR worked) - (usually someone can offer you actin primers and template at known molarity). 1. Mg concentration is key - alter this, use the temperature that gave you the best result. 2. You are using cDNA - does this mean a crude ...

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by merv
Sun Oct 02, 2011 5:00 pm
 
Forum: Molecular Biology
Topic: PLease Please I need help with my PCR! Greatly appreciated!
Replies: 8
Views: 9794

Re: Preparing buffer solns.

Hola in the case of acetic acid ,normality is the same that molarity so you can use 500x0.2=Vx17.46. Once you have the 10X TAE prepared , yes take a tent part and dilute with 9 parts of the final volume. This is a recipe of luckow and als manuals and I ...

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by protold
Mon Sep 05, 2011 5:49 am
 
Forum: Molecular Biology
Topic: Preparing buffer solns.
Replies: 6
Views: 3644
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