Molarity

Ok I believe the reason I am confused is because in chemistry when we say "concentration" we usually mean molarity, or moles per unit volume. Here you are talking about concentration in terms of mass per volume, which I thought was density? Is it different for proteins ...

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... to test all the variables (taq, dNTP's especially, to ensure the PCR worked) - (usually someone can offer you actin primers and template at known molarity). 1. Mg concentration is key - alter this, use the temperature that gave you the best result. 2. You are using cDNA - does this mean a crude ...

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... to test all the variables (taq, dNTP's especially, to ensure the PCR worked) - (usually someone can offer you actin primers and template at known molarity). 1. Mg concentration is key - alter this, use the temperature that gave you the best result. 2. You are using cDNA - does this mean a crude ...

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Hola in the case of acetic acid ,normality is the same that molarity so you can use 500x0.2=Vx17.46. Once you have the 10X TAE prepared , yes take a tent part and dilute with 9 parts of the final volume. This is a recipe of luckow and als manuals and I ...

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... 10mM EDTA Prepare: 500mL of tris (MW=121.1g), glacial acetic acid (17.46N), 0.5M EDTA Also, prepare 1L of 1xTAE. So far, I have just converted the molarity of Tris to moles and then to grams by using the MW. 0.4mol/L x 0.5L= 0.2mol Tris x 121.1g/mol= 24.22g Tris As for EDTA, I'm guessing to use ...

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