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Molar extinction coefficient

Definition

noun

(spectrophotometry)

The measure of how strongly a substance absorbs light at a particular wavelength, and is usually represented by the unit M-1cm-1 or L mol-1cm-1.


Supplement

It is symbolized by ε in Beer-Lambert law, A = εcl, where A is the absorbance of the sample, l is the pathlength (usually in centimeter), and c is the concentration of the of absorbing species in the material (moles per liter).


Synonym: molar absorptivity, molar absorption coefficient, absorbancy index, absorptivity, molar absorbancy index.
See also: spectrophotometer, absorbance.


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Optical density Q

... 35mg of X in 50 ml of water. What is the molar extinction coefficient of X at 400nm? (mol. wt. of X is 450) (20% of the marks) ...

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by monkey07
Mon Jun 01, 2009 4:34 pm
 
Forum: Molecular Biology
Topic: Optical density Q
Replies: 0
Views: 409

Molar Extinction Coefficient Trouble!

nothing. ignore it. A=EbC where E is molar extinction coefficient, b is the path of light in cm, and C is concentration

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by alexandertg6
Wed Apr 22, 2009 12:31 pm
 
Forum: Molecular Biology
Topic: Molar Extinction Coefficient Trouble!
Replies: 1
Views: 290

Molar Extinction Coefficient Trouble!

... at 614 nm. I have been asked to estimate the molar extinction coefficient for BCG from the graph and show how my value was calculated. ...

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by sophi3nutt
Tue Apr 21, 2009 11:38 am
 
Forum: Molecular Biology
Topic: Molar Extinction Coefficient Trouble!
Replies: 1
Views: 290

Re: Molar Extinction Coefficient

So if I have this data: Absorbance @ 405nm: .875 Concentration (M) of Cr(NO3)3 (aq): .25M Since you're saying that if l = 1cm (standard curvette), then E = A/C so then: .875/.25 = E E = 3.5?

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by r6barfly
Thu Mar 19, 2009 5:21 am
 
Forum: Molecular Biology
Topic: Molar Extinction Coefficient
Replies: 4
Views: 15776

Re: Beer Lambert Law

... on the power of ten? I didn't check the extinction coefficient (now preferably called the molar absorptivity) of NADH. I'll take your word for it that it is ...

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by blcr11
Thu Mar 05, 2009 2:42 pm
 
Forum: Molecular Biology
Topic: Beer Lambert Law
Replies: 1
Views: 701
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