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Moieties

(noun) Plural form of moiety.


See moiety for definition and additional information.


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Electrophoresis of ionic complexes

What about treating with paraformaldehyde? That should crosslink your 2 moieties, it can be reversed (by heating >65ÂșC) and it is quite simple (add to 0.6% v/v final concentration for 20 minutes at RT and wash by precipitating your siRNA). Then run your samples ...

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by canalon
Thu Jan 06, 2011 2:24 am
 
Forum: Molecular Biology
Topic: Electrophoresis of ionic complexes
Replies: 6
Views: 3321

Re: How do you overexpress and knock down a gene

... oligo types have different optimal delivery techniques, though any of them can be delivered into cell culture by electroporation. In-vivo delivery moieties have been developed to bring steric-blocking oligos into cells after systemic injection (e.g. i.v., i.p.) into adult animals.

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by jonmoulton
Tue Nov 30, 2010 10:27 pm
 
Forum: Molecular Biology
Topic: How do you overexpress and knock down a gene
Replies: 2
Views: 13325

Re: What are methods to promote or suppress gene expression?

... antisense oligo. Your interest in doing this in an intact animal makes it harder, as that requires systemic delivery of the oligo. There are some moieties which can be attached to a steric-blocking oligo to help the oligo enter cells (likely be enhancing endosomal escape). One example is to use ...

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by jonmoulton
Thu Jul 29, 2010 3:01 pm
 
Forum: Genetics
Topic: What are methods to promote or suppress gene expression?
Replies: 2
Views: 5266

Review: PPMO and Vivo-Morpholinos

Conjugation of Morpholino oligos with endosomal escape moieties is bringing effective antisense techniques to rodent research. Such conjugates will likely debut soon as therapeutics. This is an open-access review of two delivery-enabled forms of Morpholinos. ...

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by jonmoulton
Wed Mar 25, 2009 10:58 pm
 
Forum: Genetics
Topic: Review: PPMO and Vivo-Morpholinos
Replies: 1
Views: 1518

Re: how to knock out a gene?

... peptide-Morpholino conjugates have shown that the oligos enter cells by endocytosis and then escape from the endosome. In some way, the peptide moieties render the endosomal membrane permeable. This may be due to the combined electrostatic attraction and hydrogen-bonding of the guanidiniums ...

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by jonmoulton
Fri Oct 17, 2008 4:38 pm
 
Forum: Genetics
Topic: how to knock out a gene?
Replies: 4
Views: 7674
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