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Mobility

mobility

Capability of movement, of being moved or of flowing freely.

Origin: L. Mobilitas


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Re: SDS-PAGE

Each standard protein will be an (x,y) point on the graph where x = relative mobility and y = log(MW). Once you plot all the standard proteins, you should be able to draw a reasonable line through the points (or do a regression). This is your calibration line. ...

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by blcr11
Mon Mar 23, 2009 10:37 am
 
Forum: Molecular Biology
Topic: SDS-PAGE
Replies: 1
Views: 751

SDS-PAGE

... from bovie 66000Da and Trypsin Inhibitor from soybean 20100Da. It then goes on to say make a plot of log (molecular weight) against relative mobility for each standard protein. use the measured mobilities of the proteins in your SDS-PAGE gel, not from the picture given in our book. soo...looking ...

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by biology_06er
Mon Mar 23, 2009 3:58 am
 
Forum: Molecular Biology
Topic: SDS-PAGE
Replies: 1
Views: 751

Re:

... mixture - research conditions for binding and washing to make sure not to affect protein-DNA interactions. 7. Proceed with electrophoretic mobility shift assay AND/OR isolate DNA, clone, and sequence. 8. Let us know the outcome. Good luck! Thank you for the suggestion. I guess the only ...

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by evilid
Mon Dec 01, 2008 10:19 pm
 
Forum: Molecular Biology
Topic: Does my protein bind DNA?
Replies: 10
Views: 994

Does my protein bind DNA?

... mixture - research conditions for binding and washing to make sure not to affect protein-DNA interactions. 7. Proceed with electrophoretic mobility shift assay AND/OR isolate DNA, clone, and sequence. 8. Let us know the outcome. Good luck!

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by Cat
Sun Nov 30, 2008 9:17 pm
 
Forum: Molecular Biology
Topic: Does my protein bind DNA?
Replies: 10
Views: 994

Re:

... experiments you could do to analyze this. Beads are probably the best and the easiest, but you could also do, for example, an electrophoretic mobility shift assay. But you see that I am trying to find whether my protein ever binds to DNA. Therefore I do not know the target DNA sequence. I ...

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by evilid
Thu Nov 27, 2008 6:11 pm
 
Forum: Molecular Biology
Topic: Does my protein bind DNA?
Replies: 10
Views: 994
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