Mobility

Those affecting size on SDS-PAGE would be mostly glycosylation or proteolysis, depending on the size of your band. Or the mobility of your protein is affected for some reason. Could you measure size of your protein on MALDI MS? sorry, but I do not know what MALSI MS is.

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Those affecting size on SDS-PAGE would be mostly glycosylation or proteolysis, depending on the size of your band. Or the mobility of your protein is affected for some reason. Could you measure size of your protein on MALDI MS?

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... work on different sample) 3-there is a methylation of restriction site that blocks it for restriction enzyme. (unlikely but possible) 4-altered mobility of fragments due to wrong pH or fluorescent dye I dont know what can you see on your gel. When you load uncut plasmid and cut plasmid on the ...

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... The second possibility is that the band size does not match theroretical due to electrophoresis problem. Some dyes like sybr green alter dna mobility depending on concentration/concentration manner. Try to run your gel without any fluorecent dye and stain it after procedure.

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A Protein with His-tag may be used in a mobility shift assay?. his-tag may interact inespecifically with DNA?? by electrostatic interactions?.

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