Dictionary » M » Mobility

Mobility

mobility

Capability of movement, of being moved or of flowing freely.

Origin: L. Mobilitas


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electrophoretic mobility shift assay (EMSA)

I am doing EMSA and after the first try having success, I could never reproduce my results: following the streptavidin-biotin-HRP detection, there are no bands on the blot paper. Compared to the first succesful try, the most striking difference I experience is that while at first, there was a bromop...

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by kk
Fri Nov 08, 2013 12:21 pm
 
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 1218

Re: In Vitro Transcription Translation

... Since the protein contains the hot sulfur, you don't need a radiolabeled antibody to detect it. You can infer the mass of the protein from its mobility (location of gel band). However, if you need to unambiguously prove the identity of the protein you might need a Western as well, since your ...

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by jonmoulton
Wed Jun 05, 2013 10:59 pm
 
Forum: Molecular Biology
Topic: In Vitro Transcription Translation
Replies: 3
Views: 1975

Re:

Those affecting size on SDS-PAGE would be mostly glycosylation or proteolysis, depending on the size of your band. Or the mobility of your protein is affected for some reason. Could you measure size of your protein on MALDI MS? sorry, but I do not know what MALSI MS is.

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by Bio2013
Sat Feb 16, 2013 8:48 pm
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 3274

Western Blot

Those affecting size on SDS-PAGE would be mostly glycosylation or proteolysis, depending on the size of your band. Or the mobility of your protein is affected for some reason. Could you measure size of your protein on MALDI MS?

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by JackBean
Mon Feb 11, 2013 3:31 pm
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 3274

isolation of plasmid (Midi prep)

... work on different sample) 3-there is a methylation of restriction site that blocks it for restriction enzyme. (unlikely but possible) 4-altered mobility of fragments due to wrong pH or fluorescent dye I dont know what can you see on your gel. When you load uncut plasmid and cut plasmid on the ...

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by triton
Mon Aug 27, 2012 4:46 pm
 
Forum: Molecular Biology
Topic: isolation of plasmid (Midi prep)
Replies: 3
Views: 5190
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