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Nuclear protein purification

... protein purification I came across the step where I need to add 5M NaCl slowly with shaking until I adjust the molarity to 2M NaCl, how many mls do I need to add? Honestly I tried to tackle the problem in different ways but I couldnt, any help would be really appreciated. Thanks all.

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by bravebeaker
Mon Mar 18, 2013 7:49 am
Forum: Molecular Biology
Topic: Nuclear protein purification
Replies: 4
Views: 1682

Buffer solutions

... I can't work it out Given 1M of acetic acid and 1M NaOH You are to prepare l litre of a 0.15m acetate buffer pH is 5.0 and pKa is 4.7 1.How many mLs of hte 1M acetic acid will you use? 2.In the final buffer solution what is the concentration of acetic acid + acetate ion 3.what is the ratin of ...

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by sanzie
Mon Oct 25, 2010 1:33 am
Forum: Molecular Biology
Topic: Buffer solutions
Replies: 1
Views: 1145

Simple C1 V1 question

... dilution (100ul culture into 900ul 10mM MgSO4, blank = 100ul 2YT into 900ul 10mM MgSO4) ), read it on a spec and got .466 OD @600nm. I need 35 mls of the culture. I have to resuspend @ 1.0 OD. I need to figure the volume of buffer to resuspend after spinning down the 35 mls of culture. C1 = ...

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by shanker
Sat Oct 02, 2010 2:24 am
Forum: Microbiology
Topic: Simple C1 V1 question
Replies: 1
Views: 872

DNA CONC.. Help?

... to be 163ug/ml, by the formula 0.163x50x20=163? Or should i do it this way: 0.163x50=8.15, 8.15/300 gives 0.0271, 0.0271x1000 puts in back to mLs, giving a concentration of 27.1ug/ml; the second way apparently takes into account the concentration being ug/300mls instead of ug/ml. Please clarify.. ...

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by abbydcruz
Mon Mar 08, 2010 3:44 am
Forum: Molecular Biology
Topic: DNA CONC.. Help?
Replies: 3
Views: 1466

Re: Lab Assignment: Enzyme Catalysis ( !!! URGENT !!! )

Rise is vert (y) axis in mLs. Run is horizontal (x) axis in seconds. Answer in mL/sec. Calculate for each plot on the graph.

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by DeePDish
Wed Jan 07, 2009 5:47 pm
Forum: Cell Biology
Topic: Lab Assignment: Enzyme Catalysis ( !!! URGENT !!! )
Replies: 5
Views: 9160
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