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Minimise

minimise

To reduce to the smallest part or proportion possible. Expressed as a number, degree or extent.

To reduce to the smallest possible, to reduce to a minimum.

Origin: Minimized; Minimizimg.


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pH indicator in agar plates

... was wondering what kinds of problems I can expect using bromocresol purple indicator in the MRS agar in terms of errors and practicality so I can minimise these problems?? I plan to use 0.025ml per litre. Also, I've never used this indicator before so what can I expect in terms of the colour the ...

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by ilovebiology77
Sat Feb 25, 2012 10:35 pm
 
Forum: Microbiology
Topic: pH indicator in agar plates
Replies: 0
Views: 804

DMSO use in PCR

I am having problems with contamination so I am trying to prepare the DNA and master mix in separate areas to minimise this and UV treat all equipment and reagents that can be treated to prevent contamination. UV-treatment is only a couple of minutes in a DNA-free cabinet. The ...

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by Emmona87
Tue Dec 14, 2010 4:06 pm
 
Forum: Molecular Biology
Topic: DMSO use in PCR
Replies: 4
Views: 2617

DMSO use in PCR

Hi all, I am using DMSO in my PCRs and to minimise any contamination issues I have UV-treating my master mix components and making the master mix in a laminar cabinet before adding primers and DNA separately. I just wanted to check if it was safe ...

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by Emmona87
Tue Dec 14, 2010 3:52 pm
 
Forum: Molecular Biology
Topic: DMSO use in PCR
Replies: 4
Views: 2617

Re: Fungus growing on agar plates. contamination??

... that air is full of fungal spores. More in some places than in others. You should pour plates in a clean environment with still air, to minimise the movement of such spores. Also, open each plate only briefly. It is when you open them that spores drift in. Spores also fall off anything ...

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by skeptic
Thu Jul 08, 2010 12:31 am
 
Forum: Microbiology
Topic: Fungus growing on agar plates. contamination??
Replies: 17
Views: 13775

Re:

... for the ligation reaction? I seem to be recovering a good amount from the phenol:chloroform extractions. My advisor showed me some tricks to minimise losses). Agree about the smell and work though :) The latest thing I have done is to run a gel with the supplier's original vector straight ...

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by TaintedCherub
Fri Oct 30, 2009 7:39 pm
 
Forum: Molecular Biology
Topic: Serious cloning issues - gel extractions?
Replies: 23
Views: 20500
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