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Min recombinase

min recombinase

(Science: enzyme) member of the din family of site-specific recombinases

Registry number: EC 2.7.7.-


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Knockout mice

... mice this morning. "Knockdown of Cdc42 in the developing retina was achieved by crossing Cdc42 floxed mice [29] with mice expressing cre recombinase under the control of the α element of the Pax6 promoter [30]. " From Mat'l & Methods: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553133/

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by jonmoulton
Wed Feb 06, 2013 4:24 pm
 
Forum: Genetics
Topic: Knockout mice
Replies: 5
Views: 4551

Alignment in Site-specific Recombination

... for my exams and have a problem about site-specific recombination: When recombination between two DNA molecoules with lox sites through Cre-recombinase occurs, those two lox sites first have to come close to each other for recombination to start. How is this achieved, how do they find each ...

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by Ebson
Wed Jul 14, 2010 10:08 am
 
Forum: Genetics
Topic: Alignment in Site-specific Recombination
Replies: 0
Views: 707

What is resolvase?

... In a Holliday junction, does it cause the crossing over or does it does it cut the strands after it crosses over? And is it the same thing as recombinase? Thanks!

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by BioGradStudent
Tue Oct 21, 2008 1:09 am
 
Forum: Genetics
Topic: What is resolvase?
Replies: 0
Views: 1384

Re: Allele symbols/acronyms?

... It stands for "floxed" which itself stands for "flanked by lox P sites" the lox P sequence being the recognition site for Cre recombinase. See Kuhn, et. al. (1995) Science 269, 1427-1429. You put Cre recombinase under the control of a promoter (Mx1) which is sensitive to stimulation ...

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by blcr11
Wed Sep 10, 2008 4:20 pm
 
Forum: Genetics
Topic: Allele symbols/acronyms?
Replies: 7
Views: 9844

Re: Allele symbols/acronyms?

... while under non-permissive conditions you may see the effect of the absence of the gene’s expression. For the fl/fl cells, in the absence of Cre recombinase, Rb is present, just as if it were a normal cell. When you transfect with something that carries the recombinase, Rb is excised from both ...

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by blcr11
Wed Sep 10, 2008 1:25 am
 
Forum: Genetics
Topic: Allele symbols/acronyms?
Replies: 7
Views: 9844
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