Min

... wrong but the second signal began to appear when I diluted the second antibody 2X more and used less incubation time for the TSA reaction (about 3 min).

See entire post

... output, nausea, vomiting, fatigue and anorexia. She was diagnosed as diabetic mellitus for 10 years ago and non-regular treatment. Physical examination Vital signs: BT 37 °C (37-37.5), PR 96 /min (80-100), RR 36 /min (25-30) BP 150/90 mmHg HEENT : pale conjunctivae, neck vein engorged Heart ...

See entire post

... remember who told me this and I have been unable to find the source of this information. In fact, every protocol I can find online uses a 30-60 min recovery period following heat shock. Does anyone know a reference that documents this information or refutes it?

See entire post

Just to also add to this problem! The concentration in mM is supposed to be obtained by comparing the enzyme data with the standard curve in such a way that I convert the RFU (for the enzyme data) into mM using the free Fluorescent reference curve ( consist of mM concentrations of the Fluorescent co...

See entire post

With the fluorometric enzyme Assay how to convert the FLU/min (obtained as FlU/min slope for every concentration of substrate; serial dilutions of substrate at concentrations 200 uM to 50 uM), into to RFU/mg/min of enzyme, that is nessesary to obtain ...

See entire post