Microtome

... in "face on" view. The fresh tissue or cell suspension is frozen rapidly (cryofixed), then fractured by simply breaking or by using a microtome while maintained at liquid nitrogen temperature. The cold fractured surface (sometimes "etched" by increasing the temperature to ...

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Please check http://www.nervenet.org/main/databases.html or The Mouse Brain Library http://www.mbl.org/ where you will get tissue & microtome structures for pituitary. Because I'm not getting the Mouse brain. Thank You.

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... avenue provide an opportunity to have some notion of the type of tissue that might be developing in the on-going project. An option of using a microtome at this point was considered but it was not used for a variety of reasons, none of which will be mentioned here. As the plants developed, ...

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... :D I have never prepared a c.s or l.s of any of those type specimens. However I may attempt to do so in the future, if and when I acquire a microtome. They are quite expensive instruments, even used ones. :(

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Parafin blocks are used to have proper cuts (with microtome (not sure about its spelling) ) from animal or plant tissues. I'm trying to find my notes. I will try to give more detailed info...

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