Dictionary » M » Microtome



(Science: instrument) A device used for cutting sections from an embedded specimen, either for light or electron microscopy.

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RNA isolation from brain sections

... and then punch them is too long for good RNA isolation. Is this true? Secondly, I'd like to take fresh frozen brains that are sectioned on a microtome (20 um to 200 um) and cut out particular brain regions to extract RNA. So, here are some questions: 1) any suggested RNA isolation kits/protocols ...

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by protoemics
Fri Mar 20, 2015 2:19 am
Forum: General Discussion
Topic: RNA isolation from brain sections
Replies: 0
Views: 37

Tools for studying cells question

... in "face on" view. The fresh tissue or cell suspension is frozen rapidly (cryofixed), then fractured by simply breaking or by using a microtome while maintained at liquid nitrogen temperature. The cold fractured surface (sometimes "etched" by increasing the temperature to ...

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by MrMistery
Fri Dec 26, 2008 6:07 pm
Forum: Cell Biology
Topic: Tools for studying cells question
Replies: 2
Views: 4740

Structure of Mouse Pituitary

Please check http://www.nervenet.org/main/databases.html or The Mouse Brain Library http://www.mbl.org/ where you will get tissue & microtome structures for pituitary. Because I'm not getting the Mouse brain. Thank You.

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by dipjyoti
Mon Feb 12, 2007 10:36 am
Forum: Physiology
Topic: Structure of Mouse Pituitary
Replies: 4
Views: 5957

your answers

... avenue provide an opportunity to have some notion of the type of tissue that might be developing in the on-going project. An option of using a microtome at this point was considered but it was not used for a variety of reasons, none of which will be mentioned here. As the plants developed, ...

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Sat Mar 25, 2006 1:10 pm
Forum: Botany Discussion
Topic: Marrowfat Peas
Replies: 51
Views: 44877

Wet Preparation

... :D I have never prepared a c.s or l.s of any of those type specimens. However I may attempt to do so in the future, if and when I acquire a microtome. They are quite expensive instruments, even used ones. :(

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by Ken Ramos
Fri Dec 09, 2005 11:35 pm
Forum: Microbiology
Topic: Wet Preparation
Replies: 12
Views: 8818
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