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Fluorescence microscopy



A technique using a light microscope to study properties of organic or inorganic substances using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption.


Fluorescence microscopy is used in FISH analysis where a fluorescent probe is located if bound to specific DNA sequence that is complementary to it.

See also: FISH

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Difference between ICC and IF

I also have some doubts about fluorescence microscopy!? What technique we can use to visualise by fluorescence microscopy a transfected proteins that ...

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by simina
Tue Feb 19, 2013 9:08 pm
Forum: Molecular Biology
Topic: Difference between ICC and IF
Replies: 7
Views: 19983


What additional information can be obtained by using fluorescence microscopy over light microscopy ??

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by anamikariya
Wed Mar 21, 2012 3:33 pm
Forum: General Discussion
Topic: Microscopy
Replies: 2
Views: 2392

Calcium ions in the Cell

... cross eukaryotic cell membranes, and indictors like Fluo5N give off fluorescence when bound to these ions. When Fluo5N is introduced into the ... you expect to see fluoresce when the cells are examined by confocal microscopy? A) Mitochondria B) Lysosome C) Proteasome D) RER E) None of the ...

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by someguy456345
Sat Apr 24, 2010 3:05 pm
Forum: Cell Biology
Topic: Calcium ions in the Cell
Replies: 4
Views: 8292


... chemicals by spectrophotometric assays. you can monitor organelles by fluorescence microscopy etc. What exactly are you talking about? Your questions are so general that it is ...

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by MrMistery
Wed Aug 12, 2009 11:23 pm
Forum: Cell Biology
Topic: Photosynthesis
Replies: 10
Views: 9172

Re: Difference between ICC and IF

... processes made cells permeable). The fluorochrome is detected by fluorescence microscopy, always involving excitation of fluoresence with a defined wavelength band of light ...

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by jonmoulton
Tue Sep 16, 2008 3:37 pm
Forum: Molecular Biology
Topic: Difference between ICC and IF
Replies: 7
Views: 19983
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