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Microsatellite

microsatellite

(Science: molecular biology) short sequences of di- or trinucleotide repeats of very variable length distributed widely throughout the genome. Using pCR primers to the unique sequences upstream and downstream of a microsatellite their location and polymorphism can be determined and the technique is extensively used in investigating genetic associations with disease.


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A question regarding PCR

probably, your microsatellite is part of the chromosome, right? So there will be much more than 7 bp and it will be on both sides. Yes, the polymerase will amplify the chain until the conditions change (i.e. end of amplification ...

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by JackBean
Sat Jan 18, 2014 2:36 pm
 
Forum: Molecular Biology
Topic: A question regarding PCR
Replies: 1
Views: 455

A question regarding PCR

Hello everyone. Let's say I have this microsatellite DNA sequence: 5’-acatttg CTT CGTACACAAC TGTGCGAACA TGC caacctca tgcaacctca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca ...

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by x555
Sat Jan 18, 2014 11:05 am
 
Forum: Molecular Biology
Topic: A question regarding PCR
Replies: 1
Views: 455

microsatellite instability

Another question regarding MSI. When PCR amplifying microsatellite markers for subsequent fragment analysis by microcapillary elfo - is it important to use an ultra-high fidelity polymerase, or is it not important?

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by kk
Tue Oct 08, 2013 9:47 am
 
Forum: Molecular Biology
Topic: microsatellite instability
Replies: 2
Views: 1685

Microsatellites

Which criteria should I follow to select a couple of microsatellite markers for an haplotype determination study? Between haplotypes and within haplotypes.

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by MirrisRq
Fri May 17, 2013 6:11 pm
 
Forum: Genetics
Topic: Microsatellites
Replies: 0
Views: 485

Need help identifying these bones

I'm afraid that even if police considered it as human bones, they would perform only microsatellite test, not whole genome sequencing, which would you need to identify the species (or at least partial sequencing).

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by JackBean
Tue Dec 18, 2012 8:40 pm
 
Forum: Human Biology
Topic: Need help identifying these bones
Replies: 10
Views: 4080
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