Dictionary » M » Microsatellite

Microsatellite

microsatellite

(Science: molecular biology) short sequences of di- or trinucleotide repeats of very variable length distributed widely throughout the genome. Using pCR primers to the unique sequences upstream and downstream of a microsatellite their location and polymorphism can be determined and the technique is extensively used in investigating genetic associations with disease.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


STR analysis transfected cells

... MCF-7 cells with a pcnda Her2 vector ( I'll call these MCF7-Her2) and now just need to compare my derived cell line with the parent MCF-7 cells by microsatellite STR analysis. Since it is just an addition of one gene, should I expect the parental cell line and derived cell line to have the same ...

See entire post
by kingmcb
Thu Aug 21, 2014 11:13 pm
 
Forum: Molecular Biology
Topic: STR analysis transfected cells
Replies: 0
Views: 348

Re: microsatellite instability

OK, another question: I can see how insertion/deletion can be detected by fragment analysis, but incorporation of a wrong nucleotide wouldn't cause change in the length of amplicon, so can that also be detected by fragment analysis?

See entire post
by kk
Thu Jul 10, 2014 2:45 pm
 
Forum: Molecular Biology
Topic: microsatellite instability
Replies: 3
Views: 2521

A question regarding PCR

probably, your microsatellite is part of the chromosome, right? So there will be much more than 7 bp and it will be on both sides. Yes, the polymerase will amplify the chain until the conditions change (i.e. end of amplification ...

See entire post
by JackBean
Sat Jan 18, 2014 2:36 pm
 
Forum: Molecular Biology
Topic: A question regarding PCR
Replies: 1
Views: 1458

A question regarding PCR

Hello everyone. Let's say I have this microsatellite DNA sequence: 5’-acatttg CTT CGTACACAAC TGTGCGAACA TGC caacctca tgcaacctca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca ...

See entire post
by x555
Sat Jan 18, 2014 11:05 am
 
Forum: Molecular Biology
Topic: A question regarding PCR
Replies: 1
Views: 1458

microsatellite instability

Another question regarding MSI. When PCR amplifying microsatellite markers for subsequent fragment analysis by microcapillary elfo - is it important to use an ultra-high fidelity polymerase, or is it not important?

See entire post
by kk
Tue Oct 08, 2013 9:47 am
 
Forum: Molecular Biology
Topic: microsatellite instability
Replies: 3
Views: 2521
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 7,640 times. 
What links here | Related changes | Permanent link