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Microsatellite

microsatellite

(Science: molecular biology) short sequences of di- or trinucleotide repeats of very variable length distributed widely throughout the genome. Using pCR primers to the unique sequences upstream and downstream of a microsatellite their location and polymorphism can be determined and the technique is extensively used in investigating genetic associations with disease.


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Re: microsatellite instability

OK, another question: I can see how insertion/deletion can be detected by fragment analysis, but incorporation of a wrong nucleotide wouldn't cause change in the length of amplicon, so can that also be detected by fragment analysis?

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by kk
Thu Jul 10, 2014 2:45 pm
 
Forum: Molecular Biology
Topic: microsatellite instability
Replies: 3
Views: 2175

A question regarding PCR

probably, your microsatellite is part of the chromosome, right? So there will be much more than 7 bp and it will be on both sides. Yes, the polymerase will amplify the chain until the conditions change (i.e. end of amplification ...

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by JackBean
Sat Jan 18, 2014 2:36 pm
 
Forum: Molecular Biology
Topic: A question regarding PCR
Replies: 1
Views: 1253

A question regarding PCR

Hello everyone. Let's say I have this microsatellite DNA sequence: 5’-acatttg CTT CGTACACAAC TGTGCGAACA TGC caacctca tgcaacctca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca ...

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by x555
Sat Jan 18, 2014 11:05 am
 
Forum: Molecular Biology
Topic: A question regarding PCR
Replies: 1
Views: 1253

microsatellite instability

Another question regarding MSI. When PCR amplifying microsatellite markers for subsequent fragment analysis by microcapillary elfo - is it important to use an ultra-high fidelity polymerase, or is it not important?

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by kk
Tue Oct 08, 2013 9:47 am
 
Forum: Molecular Biology
Topic: microsatellite instability
Replies: 3
Views: 2175

Microsatellites

Which criteria should I follow to select a couple of microsatellite markers for an haplotype determination study? Between haplotypes and within haplotypes.

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by MirrisRq
Fri May 17, 2013 6:11 pm
 
Forum: Genetics
Topic: Microsatellites
Replies: 0
Views: 632
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