Dictionary » M » Microinjection



The insertion of a substance into a cell through a microelectrode. Typical applications include the injection of drugs, histochemical markers (such as horseradish peroxidase or lucifer yellow) and rNA or dNA in molecular biological studies. To extrude the substances through the very fine electrode tips, either hydrostatic pressure (pressure injection) or electric currents (ionophoresis) is employed.

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Gene knockdowns in the wasp Nasonia vitripennis

This paper describes gene knockdowns in the wasp Nasonia vitripennis by Morpholino antisense oligos, delivered by microinjection. Dual mode of embryonic development is highlighted by expression and function of Nasonia pair-rule genes. Rosenberg MI, Brent AE, Payre F, Desplan C. ...

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by jonmoulton
Fri Mar 07, 2014 5:57 pm
Forum: Molecular Biology
Topic: Gene knockdowns in the wasp Nasonia vitripennis
Replies: 0
Views: 2150

Re: Biochemical and Molecular Test

... means is called transfection. Several different transfection techniques are available, such as calcium phosphate transfection, electroporation, microinjection and liposome transfection. DNA can also be introduced into eukaryotic cells using viruses or bacteria as carriers, the latter is sometimes ...

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by sykierj
Wed Nov 16, 2011 7:12 am
Forum: Molecular Biology
Topic: Biochemical and Molecular Test
Replies: 6
Views: 3182

Re: How do you overexpress and knock down a gene

... can deliver mRNA into cells or an organism. This can be tricky as the mRNA can be degraded, but electroporation or lipofection of cell lines or microinjection into early zygotes can give useful overexpression. There are no good methods yet for organism-wide overexpression in adult animals. For ...

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by jonmoulton
Tue Nov 30, 2010 10:27 pm
Forum: Molecular Biology
Topic: How do you overexpress and knock down a gene
Replies: 2
Views: 15558

Genetic engineering - Help

... has inserted correctly 5. Establish a transfection protocol for the snail eggs (electroporation, CaCl2, chemicals like lipofectamin or fugene, microinjection…) 6. Transfect the the snail eggs with your vector (could also be a viral vector), culture the cells and select them for positive transfection ...

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by Orome
Fri May 07, 2010 8:14 am
Forum: Genetics
Topic: Genetic engineering - Help
Replies: 12
Views: 7036

Re: Calcium ions in the Cell

... into sufficient number of organelle of the same type and 3. You need a microscope which can facilitate the visualisation of the organell during microinjection process, NO MICROSCOPE OPERATING WITH UV OR VISIBLE WAVELENGTHS OF LIGHT CAN GIVE SUFFICIENT MAGNIFICATION OF THE ORGANELLE INSIDE A ...

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by nmremala
Sat May 01, 2010 7:52 am
Forum: Cell Biology
Topic: Calcium ions in the Cell
Replies: 4
Views: 8269
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