Microinjection

Video introduction to microinjection: Rosen JN, Sweeney MF, Mably JD. Microinjection of zebrafish embryos to analyze gene function. J Vis Exp. 2009 Mar 9;(25). pii: 1115. doi: 10.3791/1115. http://www.jove.com/index/details.stp?ID=1115

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... stimulation. Getting the oligos into cells is an experimental hurdle that has resulted in many different techniques, such as electroporation, microinjection, salt-shock methods such as CaCl2 shock, transfection of anionic oligo by cationic lipids such as Lipofectamine, and transfection of ...

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the calcium phosphate method is cheap, but more work. With eukaryotic cells since you have the microinjection needles, just get it under the microscope and look for the nucleus area, and inject like many many times to ensure a high yield.

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thanks guys is there an expeiment i can do in microinjection that does not require vector injection. i need this becouse the vector i orderd is going to take few weeks to arive. thanks

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Thanks are the needle of micropipette thin enough because I saw few on eBay for £10 I also found some Microinjection syringes on eBay for £20 but the needles are about £20 for 2 from http://www.med1online.com but if micropipette does the job what the heck. any ideas on were ...

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