Mice

... am using transgenic mouse where my gene of interest is floxed. When I cross this mouse line with Tamoxifen inducible Cre line, CAGGCre-ERTM, I get mice with with heterozygous Cre and floxed gene of interest. The experimental plan was to isolate myoblasts from these mice and induce Cre in cell culture ...

See entire post

I ran across a paper with the genetic version of knockdown mice this morning. "Knockdown of Cdc42 in the developing retina was achieved by crossing Cdc42 floxed mice [29] with mice expressing cre recombinase under the control of the α element of ...

See entire post

"Does this imply that all the somatic cells in the mice contain 1 copy of the functional gene and 1 copy of the non-functional gene? " Yes. "Would this then mean that each cell is expressing the protein to some extent?" Likely yes, ...

See entire post

Thanks for your response! Does this imply that all the somatic cells in the mice contain 1 copy of the functional gene and 1 copy of the non-functional gene? Would this then mean that each cell is expressing the protein to some extent? What confuses me is that ...

See entire post

Can someone please explain how it is possible to have a heterozygous knock out mouse? If a gene is "knocked-out," how can the mouse be heterozygous for the gene? Will the mouse still produce express this "knocked-out" gene? Thanks very much!

See entire post