Methylated

Methylation of histones. The allele is wrapped around the histones, and when their tails are methylated, the histones stay closely condense and do not let any transcription factors bind to their elements for transcription. So you can have the allele, but it won't be expressed: ...

See entire post

... is of the same size as the plasmid. Therefore, the template DNA must be eliminated by enzymatic digestion with a restriction enzyme specific for methylated DNA. The template, which for this technique should be biosynthesized will be digested, but the mutated plasmid is preserved because it was ...

See entire post

I think the gene gets methylated, not sure though, you will have to check it. As to what tells the body that the recessive allele needs to be silenced its a mystery to me.

See entire post

you might want to include "DNA is not methylated", because that is the strategy bacteria use to hide their DNA from endonucleases. Other than that, you're all set

See entire post

... Don’t have one. I’ve never resorted to RE digestion to remove unwanted DNA. The Stratagene site-directed mutagenesis kits take advantage of methylated vs non-methylated DNA to selectively digest non-mutated DNAs with DpnI which greatly enriches the products for mutants, but that’s the closest ...

See entire post