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Methylated

methylated

(Science: chemistry) Impregnated with, or containing, methyl alcohol or wood spirit; as, methylated spirits.


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Genomic Imprinting In Humans (and classical genetics)

Methylation of histones. The allele is wrapped around the histones, and when their tails are methylated, the histones stay closely condense and do not let any transcription factors bind to their elements for transcription. So you can have the allele, but it won't be expressed: ...

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by kolean
Mon Jul 20, 2009 9:12 pm
 
Forum: Genetics
Topic: Genomic Imprinting In Humans (and classical genetics)
Replies: 7
Views: 347

Uses for the PCR technique?

... is of the same size as the plasmid. Therefore, the template DNA must be eliminated by enzymatic digestion with a restriction enzyme specific for methylated DNA. The template, which for this technique should be biosynthesized will be digested, but the mutated plasmid is preserved because it was ...

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by MrMistery
Mon May 04, 2009 11:41 pm
 
Forum: Genetics
Topic: Uses for the PCR technique?
Replies: 4
Views: 1349

Dominance - how exactly does it come about?

I think the gene gets methylated, not sure though, you will have to check it. As to what tells the body that the recessive allele needs to be silenced its a mystery to me.

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by stopherlogic
Thu Nov 13, 2008 8:35 am
 
Forum: Genetics
Topic: Dominance - how exactly does it come about?
Replies: 3
Views: 636

calculation: frequency of recognition site restricted by RE

you might want to include "DNA is not methylated", because that is the strategy bacteria use to hide their DNA from endonucleases. Other than that, you're all set

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by MrMistery
Mon Oct 20, 2008 7:22 pm
 
Forum: Molecular Biology
Topic: calculation: frequency of recognition site restricted by RE
Replies: 3
Views: 740

Re: problem with cloning/restriction digest

... Don’t have one. I’ve never resorted to RE digestion to remove unwanted DNA. The Stratagene site-directed mutagenesis kits take advantage of methylated vs non-methylated DNA to selectively digest non-mutated DNAs with DpnI which greatly enriches the products for mutants, but that’s the closest ...

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by blcr11
Fri Feb 22, 2008 11:40 pm
 
Forum: Molecular Biology
Topic: problem with cloning/restriction digest
Replies: 35
Views: 9012
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