Methyl

Yes, that sounds too low, at least for those wavelengths where methyl red absorbs strongly. Did you correct your concentrations for the dilution you made as you mixed your stock solution into liquid prior to making the spectrometer measurement?

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... back to the raw data on a graph and make sure the slope makes sense given the graph you see. By the way, using the above i got 0.01871m-1 cm-1 for methyl red....is this value not too small for the molar extinction coefficient though?

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don't you need more values? oh yeah sorry and methyl red concentration was 12.5um....3ml of buffer used and 1ml of stock methyl red..if that helps?...really cant get my head round this..i've tried searching

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... NADPH-dependent flavoprotein. Microbiology,151, 1433-1441. This is: a. A good reference for both the pKa and molar extinction coefficient of methyl red at 500nm b. A reference for the molar extinction coefficient of the acid form of methyl red but it isn't exactly at 500nm c. A good reference ...

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... attenuated endoplasmic reticulum stress in adipose tissue. Cant find as much literature on it as i expected http://en.wikipedia.org/wiki/Trimethylglycine TMG is an important cofactor in methylation, a process that occurs in every cell of mammals to synthesize and donate methyl groups (CH3) ...

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