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Taqman probe design using FISH probe (need help)

... have different characteristics. Probes for FISH need to be at least 1 kb long, to hybridize and "highlight" the target chromosomal DNA (metaphase chromosomes or interphase nuclei). Hydrolysis probes (such as Taqman) are very short and used in combination with other two oligonucleotides. ...

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by protoemics
Wed Feb 04, 2015 8:11 am
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 582

independent assortment

... sister chromatids are no longer identical so diversity should be increased during meiosis II because of independent assortment of chromosomes in metaphase II, conserning the recombinant and non-recombinant sister chromatids. Am I right or is there more to this story? Why is this never mentioned ...

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by leeroykincaid
Fri Apr 12, 2013 7:28 am
Forum: Cell Biology
Topic: independent assortment
Replies: 2
Views: 5776

Re: Mitosis and meiosis!

... of chromosomes number compared parent cell. In Meiosis Prophase I is different from Prophase in mitosis where there is crossing over occur. In Metaphase I, the homologous chromosomes are aligned at the equatorial line while metaphase in mitosis not. In Anaphase I, the homologous chromosomes ...

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by mavis
Wed Jan 30, 2013 9:00 am
Forum: Cell Biology
Topic: Mitosis and meiosis!
Replies: 3
Views: 5089

Re: Crossing Over

I have a prof that says that crossing over in meiosis happens during metaphase 1. I asked her about this, pointing out the evidence that I had that said that crossing over happens during prophase 1. She says that the line between prophase and metaphase are ...

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by LadyAmalara
Thu Oct 25, 2012 12:43 am
Forum: Genetics
Topic: Crossing Over
Replies: 12
Views: 26386

Re: Genetics Homeworkkk!!! PLEASE HELP

... from his mother. Using the Genotype of this man, and only considering chromosomes #1, X, and Y, draw the correct configuration of chromosomes for Metaphase of Mitosis and one possibility for Metaphase of Meiosis 1. Shade in the paternal chromosomes and label the appropriate chromatid with the ...

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by david23
Fri Sep 14, 2012 11:03 pm
Forum: Genetics
Topic: Genetics Homeworkkk!!! PLEASE HELP
Replies: 5
Views: 5154
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