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Metalloproteins

metalloproteins

proteins that have one or more tightly bound metal ions forming part of their structure.


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Re: inactive enzyme after purification with Ni-TED

There are a number of potential problems with nickel chelating resins and metalloproteins. First, the background buffer is typically phosphate which can form soluble and insoluble complexes with metals, leaching them off the protein in the process. Second, imidazole ...

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by blcr11
Sat May 03, 2008 9:32 pm
 
Forum: Molecular Biology
Topic: inactive enzyme after purification with Ni-TED
Replies: 3
Views: 2520


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