Dictionary » M » Melting temperature of dna

Melting temperature of dna

melting temperature of DNA --> denaturation temperature of DNA

That temperature at which, under a given set of conditions, double-stranded dNA is changed (50%) to single-stranded DNA; under standard conditions, the base composition of the DNA can be estimated from the denaturation temperature, since the greater the denaturation temperature, the greater the guanine-plus-cytosine content (i.e., gC content) of the DNA.

Synonym: melting temperature of DNA.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Formation of helices in heterogeneous protein vs. homogeneou

... Relate it to the coil-helix transition, fractional helicity and melting temperature.

See entire post
by JordanGo
Fri Oct 19, 2012 8:16 pm
 
Forum: Cell Biology
Topic: Formation of helices in heterogeneous protein vs. homogeneou
Replies: 0
Views: 1198

Primers Tm

Your primer melting temps for each set are just too far apart. Just consider that at say annealing temp ... 2x of primer 1 (low Tm):1x of primer 2 (high Tm) and running increasing annealing temperature range. Go from 59 to 68 in say 0.5 degree increase per cycle. Let me know what ...

See entire post
by Cat
Fri Oct 12, 2012 1:14 am
 
Forum: Molecular Biology
Topic: Primers Tm
Replies: 7
Views: 2953

Re: PCR: Low Primer Annealing Temperature Explanation?

A bit more detail. Three things: 1. Strands of DNA stick to each other through hydrogen bonding. ... determined by the amount of thermal energy (temperature). To understand what happens in a PCR ... DNA is a population of molecules (after melting of the template DNA). Any one molecule of ...

See entire post
by wbla3335
Sat Oct 06, 2012 2:30 pm
 
Forum: Genetics
Topic: PCR: Low Primer Annealing Temperature Explanation?
Replies: 4
Views: 9400

PCR: Low Primer Annealing Temperature Explanation?

That temperature is called Tm which stands for melting temperature. Tm is a temperature at which 50% of primer is free and 50% bound to the template. Since each ...

See entire post
by Cat
Sat Oct 06, 2012 1:26 pm
 
Forum: Genetics
Topic: PCR: Low Primer Annealing Temperature Explanation?
Replies: 4
Views: 9400

Re: general QPCR problem

... (how long, what GC content, what predicted melting temperature. you should have considered all of this - also ... (have you looked at the genomic sequence and the cDNA) , and when you say your gene is not up without treatment ...

See entire post
by merv
Sun Oct 02, 2011 4:10 pm
 
Forum: Molecular Biology
Topic: general QPCR problem
Replies: 9
Views: 7800
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 13,401 times. 
What links here | Related changes | Permanent link