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Melting temperature of dna

melting temperature of DNA --> denaturation temperature of DNA

That temperature at which, under a given set of conditions, double-stranded dNA is changed (50%) to single-stranded DNA; under standard conditions, the base composition of the DNA can be estimated from the denaturation temperature, since the greater the denaturation temperature, the greater the guanine-plus-cytosine content (i.e., gC content) of the DNA.

Synonym: melting temperature of DNA.


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Re: PCR: Low Primer Annealing Temperature Explanation?

A bit more detail. Three things: 1. Strands of DNA stick to each other through hydrogen bonding. ... determined by the amount of thermal energy (temperature). To understand what happens in a PCR ... DNA is a population of molecules (after melting of the template DNA). Any one molecule of ...

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by wbla3335
Sat Oct 06, 2012 2:30 pm
 
Forum: Genetics
Topic: PCR: Low Primer Annealing Temperature Explanation?
Replies: 4
Views: 6403

Re: general QPCR problem

... (how long, what GC content, what predicted melting temperature. you should have considered all of this - also ... (have you looked at the genomic sequence and the cDNA) , and when you say your gene is not up without treatment ...

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by merv
Sun Oct 02, 2011 4:10 pm
 
Forum: Molecular Biology
Topic: general QPCR problem
Replies: 9
Views: 7128

QuestionS on Taxonomy

... to get more opinion on my choice, thanx. The melting temperature from two gram positive bacteria was determined ... a species? 1. Gram positive, melting temperature of DNA within 5 C and filamentous growth 2. rRNA with less ...

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by klapausius
Fri Oct 08, 2010 1:32 pm
 
Forum: Microbiology
Topic: QuestionS on Taxonomy
Replies: 1
Views: 3911

DNA CONC.. Help?

look for DNA denaturation and melting temperature (of DNA) alias T_m (m is subscript)

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by JackBean
Mon Mar 08, 2010 11:22 am
 
Forum: Molecular Biology
Topic: DNA CONC.. Help?
Replies: 3
Views: 1371

maping and linkage problems

... cell, what is the weight (in ╬╝grams) of this DNA? c. If you extracted chromosomal DNA from a ... circumstances influence characteristics of temperature-induced DNA melting. a. Percentage of GC base pairs b. Urea (forms hydrogen ...

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by hemu0912
Sun Jul 26, 2009 5:59 pm
 
Forum: Genetics
Topic: maping and linkage problems
Replies: 3
Views: 6273
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