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Melting temperature

melting temperature --> temperature midpoint

The midpoint in the change in optical properties (absorbance, rotation) of a structured polymer (e.g., dNA) with increasing temperature.

Synonym: melting temperature.


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Formation of helices in heterogeneous protein vs. homogeneou

... Relate it to the coil-helix transition, fractional helicity and melting temperature.

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by JordanGo
Fri Oct 19, 2012 8:16 pm
 
Forum: Cell Biology
Topic: Formation of helices in heterogeneous protein vs. homogeneou
Replies: 0
Views: 1127

Primers Tm

Your primer melting temps for each set are just too far apart. Just consider that at say annealing temp ... 2x of primer 1 (low Tm):1x of primer 2 (high Tm) and running increasing annealing temperature range. Go from 59 to 68 in say 0.5 degree increase per cycle. Let me know what ...

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by Cat
Fri Oct 12, 2012 1:14 am
 
Forum: Molecular Biology
Topic: Primers Tm
Replies: 7
Views: 2777

Re: PCR: Low Primer Annealing Temperature Explanation?

... at rates and frequencies determined by the amount of thermal energy (temperature). To understand what happens in a PCR reaction, it is helpful ... and each strand of the template DNA is a population of molecules (after melting of the template DNA). Any one molecule of primer may stick anywhere ...

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by wbla3335
Sat Oct 06, 2012 2:30 pm
 
Forum: Genetics
Topic: PCR: Low Primer Annealing Temperature Explanation?
Replies: 4
Views: 8252

PCR: Low Primer Annealing Temperature Explanation?

That temperature is called Tm which stands for melting temperature. Tm is a temperature at which 50% of primer is free and 50% bound to the template. Since each ...

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by Cat
Sat Oct 06, 2012 1:26 pm
 
Forum: Genetics
Topic: PCR: Low Primer Annealing Temperature Explanation?
Replies: 4
Views: 8252

Re: general QPCR problem

... do not bind non-specifically (how long, what GC content, what predicted melting temperature. you should have considered all of this - also have you run the primers through ...

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by merv
Sun Oct 02, 2011 4:10 pm
 
Forum: Molecular Biology
Topic: general QPCR problem
Replies: 9
Views: 7593
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