Melting temperature

... Relate it to the coil-helix transition, fractional helicity and melting temperature.

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Your primer melting temps for each set are just too far apart. Just consider that at say annealing temp ... 2x of primer 1 (low Tm):1x of primer 2 (high Tm) and running increasing annealing temperature range. Go from 59 to 68 in say 0.5 degree increase per cycle. Let me know what ...

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... at rates and frequencies determined by the amount of thermal energy (temperature). To understand what happens in a PCR reaction, it is helpful ... and each strand of the template DNA is a population of molecules (after melting of the template DNA). Any one molecule of primer may stick anywhere ...

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That temperature is called Tm which stands for melting temperature. Tm is a temperature at which 50% of primer is free and 50% bound to the template. Since each ...

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... do not bind non-specifically (how long, what GC content, what predicted melting temperature. you should have considered all of this - also have you run the primers through ...

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