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Melting

melting

liquefaction; the act of causing (something) to melt, or the process of becoming melted.

(Science: chemistry) Melting point, the degree of temperature at which a solid substance melts or fuses; as, the melting point of ice is 0 deg centigrade or 32 deg Fahr, that of urea is 132 deg Centigrade. Melting pot, a vessel in which anything is melted; a crucible.


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Basics for preparing bacterial cultures

... However, I would disagree about the microwave, we use it standardly for melting agarose and agar should work as well. One just has to check that it doesn't boil. It's always good to mix the bottle during heating. By that ...

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by JackBean
Sun Nov 25, 2012 11:13 pm
 
Forum: Microbiology
Topic: Basics for preparing bacterial cultures
Replies: 4
Views: 957

Tm of Human DNA ?

Not sure what you want, but look at this: http://www.biophp.org/minitools/melting_temperature/demo.php?formula=basic

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by Cat
Fri Oct 19, 2012 11:12 pm
 
Forum: Molecular Biology
Topic: Tm of Human DNA ?
Replies: 3
Views: 523

Formation of helices in heterogeneous protein vs. homogeneou

... protein, other than the propensity for helices being sequence dependent. Relate it to the coil-helix transition, fractional helicity and melting temperature.

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by JordanGo
Fri Oct 19, 2012 8:16 pm
 
Forum: Cell Biology
Topic: Formation of helices in heterogeneous protein vs. homogeneou
Replies: 0
Views: 454

Primers Tm

Your primer melting temps for each set are just too far apart. Just consider that at say annealing temp of 59, only 50% of one primer is bound to template and ALL (or close to 100%) of the second primer is bound non-specifically ...

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by Cat
Fri Oct 12, 2012 1:14 am
 
Forum: Molecular Biology
Topic: Primers Tm
Replies: 7
Views: 870

Re: PCR: Low Primer Annealing Temperature Explanation?

... as populations of molecules. Each primer is a population of molecules, and each strand of the template DNA is a population of molecules (after melting of the template DNA). Any one molecule of primer may stick anywhere on a molecule of template DNA whenever hydrogen bonds can form. The duration ...

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by wbla3335
Sat Oct 06, 2012 2:30 pm
 
Forum: Genetics
Topic: PCR: Low Primer Annealing Temperature Explanation?
Replies: 4
Views: 1143
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