Dictionary » M » Melting

Melting

melting

liquefaction; the act of causing (something) to melt, or the process of becoming melted.

(Science: chemistry) Melting point, the degree of temperature at which a solid substance melts or fuses; as, the melting point of ice is 0 deg centigrade or 32 deg Fahr, that of urea is 132 deg Centigrade. Melting pot, a vessel in which anything is melted; a crucible.


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qPCR vs Northern Blot

... result by qPCR - and then surprise - no silencing (or max around 20% efficiency). Primers had efficiency checked, and qPCR had no contamination, melting curve looked fine etc. Then I ask you - which result should I believe? Of course I'm going to repeat this, so far I just did technical repetition ...

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by Nevthonien
Thu Nov 07, 2013 8:35 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 2578

Asking for help with electrophoresis

... more gel electrophoresis and got quite good results this time. Below was what I changed: 1. 1.8% agarose gel, but still regular agarose 2. When melting the agarose, instead of using microwave, this time I used a heating block with stirring. 3. Used a much bigger electrophoresis tank with buffer ...

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by ll2013
Mon Jun 17, 2013 1:51 pm
 
Forum: Molecular Biology
Topic: Asking for help with electrophoresis
Replies: 5
Views: 2227

Basics for preparing bacterial cultures

... However, I would disagree about the microwave, we use it standardly for melting agarose and agar should work as well. One just has to check that it doesn't boil. It's always good to mix the bottle during heating. By that ...

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by JackBean
Sun Nov 25, 2012 11:13 pm
 
Forum: Microbiology
Topic: Basics for preparing bacterial cultures
Replies: 4
Views: 3246

Tm of Human DNA ?

Not sure what you want, but look at this: http://www.biophp.org/minitools/melting_temperature/demo.php?formula=basic

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by Cat
Fri Oct 19, 2012 11:12 pm
 
Forum: Molecular Biology
Topic: Tm of Human DNA ?
Replies: 3
Views: 1256

Formation of helices in heterogeneous protein vs. homogeneou

... protein, other than the propensity for helices being sequence dependent. Relate it to the coil-helix transition, fractional helicity and melting temperature.

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by JordanGo
Fri Oct 19, 2012 8:16 pm
 
Forum: Cell Biology
Topic: Formation of helices in heterogeneous protein vs. homogeneou
Replies: 0
Views: 1145
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