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(Science: zoology) See milt.

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amplification in no RT control

... all of my no RT controls (4 treatments and an untreated), although this is occurring ~20 cycles later than RT+ samples. I am getting single peak melt curves and my NTC is not amplifying. This suggests to me that I am not getting dimers and my reagents are 'good' I have tried cDNA at 10ng and ...

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by biomart
Fri Mar 20, 2015 1:58 am
Forum: General Discussion
Topic: amplification in no RT control
Replies: 1
Views: 45

Dioxin and Heated Bottled Water

... is just as dangerous when placed over foods to be cooked in the microwave. As the food is nuked, the high heat causes poisonous toxins to actually melt out of the plastic wrap and drip into the food. Cover food with a paper towel instead. This is an article that should be share to anyone important ...

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by Ahsmeah
Fri Aug 02, 2013 3:21 pm
Forum: General Discussion
Topic: Dioxin and Heated Bottled Water
Replies: 1
Views: 2183

Sound and how the brain reacts to it

... Here's my list of triggers: - Libraries. If someone is re-shelving or flipping through pages, I have to stop what I'm doing, just stand there and melt. - Newspapers. Like others, I also discovered this caused the same feeling so I would deliberately sit near my Grandpa as he flipped through the ...

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by Soundstrue
Fri Dec 14, 2012 4:22 pm
Forum: Human Biology
Topic: Sound and how the brain reacts to it
Replies: 89
Views: 175763

Basics for preparing bacterial cultures

... colorful bottles are there only for advertisement. You have How-To video even on that page http://www.carolina.com/teacher-resources/Video/how-to-melt-and-pour-agar-plates-video/tr11194.tr However, I would disagree about the microwave, we use it standardly for melting agarose and agar should work ...

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by JackBean
Sun Nov 25, 2012 11:13 pm
Forum: Microbiology
Topic: Basics for preparing bacterial cultures
Replies: 4
Views: 3895

PCR: Low Primer Annealing Temperature Explanation?

... the target. This is due to generation of amplicons from non-specific primer annealing when this step is performed at temperature insufficient to melt such mismatched pairs."

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by Cat
Sun Oct 07, 2012 4:16 pm
Forum: Genetics
Topic: PCR: Low Primer Annealing Temperature Explanation?
Replies: 4
Views: 15686
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