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Medium

medium

(Science: cell biology) shorthand for culture medium or growth medium, the nutrient solution in which cells or organs are grown.


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Re: sterilization in microwave oven

... did not do more than wash instruments in soap and water. The method of 'inspissation' came into use after the turn of the 20th century. A culture medium would be heated to boiling and then held at room temperature or even incubated for a few hours to a day or two. The heat killed all vegetative ...

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by fdgsr
Tue Mar 18, 2014 3:13 pm
 
Forum: Microbiology
Topic: sterilization in microwave oven
Replies: 29
Views: 93667

The effect of ethanol on peroxidase activity

... denature proteins, but at higher concentrations 2) you added the ethanol to yest, not to proteins 3) what was in that solution? Was it some growth medium or was it just water/buffer/whatever?

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by JackBean
Mon Feb 17, 2014 12:43 pm
 
Forum: General Discussion
Topic: The effect of ethanol on peroxidase activity
Replies: 1
Views: 214

Re: centrifugation of hemocytes

... pellet, BUT today it tried again, after 24h of incubation (cells have time to adhere), and centrifugation at 400 for 5 min, I gently took off the medium and resuspend the cells by pipetting thoroughly up/down: the viability worked well. However, that time i skiped the washing step (and wait 24h...), ...

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by Thom
Tue Jan 28, 2014 4:16 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 631

centrifugation of hemocytes

... and so when i wash, the cells should stay sticked, unless i resuspend with PBS containing EDTA or heparin... Is it correct? When i take off the medium after centrifugation, and then add PBS are the cell straightforward resuspended or can I take the new added fresh PBS off again until I really ...

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by Thom
Tue Jan 28, 2014 10:50 am
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 631

fluoroscopy

... technique is based on differential penetration of X-rays into different tissues. b)Blood vessels can be highlighted without the need of a contrast medium. c)Requires subtraction techniques to produce a clear image of blood vessels. d)It does not allow constant imaging of blood vessels as interventional ...

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by zak
Sat Dec 28, 2013 4:43 pm
 
Forum: Human Biology
Topic: fluoroscopy
Replies: 1
Views: 573
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