Measurement

If you don't mind losing the flexibility of scanning the spectrum or rapidly changing ex/em bands, you would build a 2-band fluorescence colorimeter. You'd need two emission filters and one or two excitation filters, arranged around your cuvette so that the excitation and emission light paths inters...

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Hi， In the lab，I use fluorescence spectrometer for the ratiometric fluorescence measurement which needs to measure optical intensity at two different wavelenghts. However, the fluorescence spectrometer is expensive and huge. I am wondering how to build a portable ...

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... Did you correct your concentrations for the dilution you made as you mixed your stock solution into liquid prior to making the spectrometer measurement?

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... in those units should be tarred and feathered). The concentration you get from Beer's law is the concentration in the cell at the time the measurement was made. You need to calculate back to the concentration of the original sample in 50ul. That's a pretty straightforward dilution calculation. ...

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maybe you could use fehling's solution and measure color change using a spectrophotometer? There may or may not be conversions for the measurement you get; you'd have to look it up. Then you could look up the ratio of glucose to monosaccharides for the plant you're using, and then from those ...

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