Mean vector

When there is no glucose in the media (-Glu) then the promoter will turn on and express your vector, which in this case is the cdc25. It will over produce this dephosphatase and the MPF will be active faster, which will push the cell cycle faster. It will push the cell ...

See entire post

... and even when you take them out of the freezer you should keep them on ice or even better use an enzyme cooler. 2) Theoretically you're right: the vector shouldn't re-ligate. Unfortunately, in reality it does re-ligate, and sometimes with quite good efficiency (the reason is mainly that bases can ...

See entire post

... then how can it go bad when left at RT? 2)Since I used two different restriction enzymes producing overhangs of different bases how can the vector religate, in other words how would the CIP help, wouldnt using CIP make more sense in blunt end producing enzymes? I had followed points 4 and ...

See entire post

Hello, I have two problems: 1) We ordered pOTB7 vector with gene A from a company and constructed deletion constructs of the gene A from above vector. We designed primers with restriction sites for 4 deletion constructs, PCR amplified and gel purified ...

See entire post

Hello, I have two problems: 1) We ordered pOTB7 vector with gene A from a company and constructed deletion constructs of the gene A from above vector. We designed primers with restriction sites for 4 deletion constructs, PCR amplified and gel purified ...

See entire post