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Mean temperature

mean temperature

The average atmospheric temperature in any locality for a designated period of time, as a month or a year.


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Re: sterilization in microwave oven

... The method of 'inspissation' came into use after the turn of the 20th century. A culture medium would be heated to boiling and then held at room temperature or even incubated for a few hours to a day or two. The heat killed all vegetative forms, and the spores germinated to vegetative forms. ...

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by fdgsr
Tue Mar 18, 2014 3:13 pm
 
Forum: Microbiology
Topic: sterilization in microwave oven
Replies: 29
Views: 93686

Transpiration and Photosynthesis.

... left open a much greater volume of water can be lose this way. You can therefore assume factors that increase the rate of photosynthesis (higher temperature, light intensity etc.) will also increase the rate of transpiration.

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by Babybel56
Fri Mar 14, 2014 4:07 am
 
Forum: Botany Discussion
Topic: Transpiration and Photosynthesis.
Replies: 3
Views: 226

Re: Humans Natural Habitat/Optimal Environment

... BARNES & NOBLE BOOKS; NEW YORK, NY; 1994; p031 Man may be considered a tropical animal because when he is naked and unprotected, the temperature of his surroundings must be 82.4°F (28°C) for his metabolism to be maintained at a minimum base level, and for him to remain in thermal ...

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by Gannet
Wed Mar 05, 2014 11:31 pm
 
Forum: Evolution
Topic: Humans Natural Habitat/Optimal Environment
Replies: 2
Views: 246

Humans Natural Habitat/Optimal Environment

... the continent, but evolution did not stop after we left. As you mentioned your height and physical stature may make you better suited to warmer temperatures, but that isn't to say that your interior metabolism would be happiest here. Though there is a narrow band of temperatures/humidities etc. ...

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by Babybel56
Tue Mar 04, 2014 6:04 am
 
Forum: Evolution
Topic: Humans Natural Habitat/Optimal Environment
Replies: 2
Views: 246

Annealing Temparature

... matching part is quite short. I would anneal the first cycle at 50°C and the rest at 65°C. Denaturation for 10s will be enough, but a bit higher temperature 95-98°C might be better. Elongation depends on polymerase, so 1 min can be enough for Phusion, but 4 min will be required for Pfu.

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by vladCH
Sat Feb 22, 2014 7:54 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 350
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