Maximum temperature

... I tested the effect of glycerol on the viability of the tissues at room temperature (2), and added a positive control (only tissue in culture medium) ... 490 as described in the kit. The absorbance in (1), (2) and (3) had a maximum after 1h and then went down again. (3) had an average abs. of 0.5 ...

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... blooded animal). Graphs that display that metabolic rates rise when temperature rises is a bit another thing. Thermal inertia, afaik, is a simple ... if they had high metabolic rates. I think there is even a theoretical maximum size that an organism can become before overheating becomes a problem. ...

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Cat, yes I decreased the annealing temperature from 55-50 and it still didn't work. I'll suggest increasing the MgCl2 ... thanks for that advice. Mith I already run 30 cycles and I read that is the maximum under standard conditions.

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... the intensity of the light getting through the blue colour. Find the maximum light intensity (it will be when all the yeast is dead) and the temperature at that point is your answer.

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... the intensity of the light getting through the blue colour. Find the maximum light intensity (it will be when all the yeast is dead) and the temperature at that point is your answer. Any suggestions to improve this plan will be appreciated

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