Dictionary » M » Maxam gilbert method

Maxam gilbert method

Maxam Gilbert method

A method of dNA sequencing, based on the controlled degradation of a dNA fragment in a set of independent, nucleotide specific reactions. The resulting fragments have characteristic sizes depending on the sequence of the template, that can be resolved on a sequencing gel. Although no longer the main protocol, Maxam gilbert sequencing still has advantages, for example for oligonucleotides or covalently modified DNA.

See: dideoxy sequencing.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Sequencing Primers

... be a good question. There are several possible methods for identifying the bases of an unknown ... identifies the presence of the known sequence. Maxam-Gilbert sequencing provides another possibility. In this ...

See entire post
by DougB
Mon Apr 23, 2012 6:25 pm
 
Forum: Genetics
Topic: Sequencing Primers
Replies: 4
Views: 3185

sequencing

hi friends, I know some of methods for Sanger dna sequencing, maxam gilbert sequencing, pyrosequencing, automated dna sequencing which are ...

See entire post
by kanagasundar
Thu Dec 09, 2010 3:48 pm
 
Forum: Molecular Biology
Topic: sequencing
Replies: 6
Views: 2292

sanger sequencing

... has been achieved using the chain termination method [1], developed by Frederick Sanger and Howard ... to deliver large amounts. In 1976-7, Allan Maxam and Walter Gilbert developed a method of DNA sequencing based on chemical ...

See entire post
by CyLMH
Mon Mar 19, 2007 1:50 pm
 
Forum: Molecular Biology
Topic: sanger sequencing
Replies: 2
Views: 3368


This page was last modified 21:16, 3 October 2005. This page has been accessed 3,862 times. 
What links here | Related changes | Permanent link