Mammalian expression vector

... off, how should I go about getting a suitable expression vector? I.e., what should I be looking for? I know that the vector should have a mammalian selection marker, and most mammalian expression vectors ...

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... that you need to reclone the same cDNA into a mammalian expression vector, which has unique BlgII and BamHI sites. How would you do ...

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... that you need to reclone the same cDNA into a mammalian expression vector, which has unique BlgII and BamHI sites. How would you do ...

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... a hint. T7: Inducible promoter for bacterial expression (e.g. pET system), needs T7 polymerase ... the lac-operon) CMV: constitutive promoter for mammalian expression Do you have a shuttle vector for both bacterial and mammalian expression? The ...

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... you're right. We are shuttling from an E.coli expression vector (for propagation) to a mammalian expression vector--or at least that's the plan. I think ...

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