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Mammalian expression vector

Definition

noun

A type of expression vector that produces significant amounts of eukaryotic protein.


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cDNA cloning and Lentiviral transfection

... my research. My ultimate aim is to transfect mammalian cells so that they have integrin labelled ... mcherry labelled integrin cDNA in a mammalian expression vector. If my understanding is correct, I can transfect ...

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by rkrishna
Thu Jun 27, 2013 11:22 pm
 
Forum: Molecular Biology
Topic: cDNA cloning and Lentiviral transfection
Replies: 1
Views: 911

How to go about creating a stably-transfected cell line

... off, how should I go about getting a suitable expression vector? I.e., what should I be looking for? I know that the vector should have a mammalian selection marker, and most mammalian expression vectors ...

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by JustinNg
Mon Sep 17, 2012 9:26 am
 
Forum: Molecular Biology
Topic: How to go about creating a stably-transfected cell line
Replies: 1
Views: 1859

Question

... that you need to reclone the same cDNA into a mammalian expression vector, which has unique BlgII and BamHI sites. How would you do ...

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by dor940
Mon Dec 07, 2009 1:40 am
 
Forum: Cell Biology
Topic: Question
Replies: 1
Views: 700

Protocol Question

... that you need to reclone the same cDNA into a mammalian expression vector, which has unique BlgII and BamHI sites. How would you do ...

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by dor940
Sun Dec 06, 2009 11:06 pm
 
Forum: Molecular Biology
Topic: Protocol Question
Replies: 1
Views: 805

Please explain to me - CMVp

... a hint. T7: Inducible promoter for bacterial expression (e.g. pET system), needs T7 polymerase ... the lac-operon) CMV: constitutive promoter for mammalian expression Do you have a shuttle vector for both bacterial and mammalian expression? The ...

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by Duke
Tue Oct 02, 2007 11:37 am
 
Forum: Molecular Biology
Topic: Please explain to me - CMVp
Replies: 4
Views: 3705
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