Mammalian expression vector

... a hint. T7: Inducible promoter for bacterial expression (e.g. pET system), needs T7 polymerase ... the lac-operon) CMV: constitutive promoter for mammalian expression Do you have a shuttle vector for both bacterial and mammalian expression? The ...

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... you're right. We are shuttling from an E.coli expression vector (for propagation) to a mammalian expression vector--or at least that's the plan. I think ...

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... some kind of cassette-style transfers to other vectors. It does rely on methylated/unmethylated ... between an E coli vector for propagation and a mammalian expression vector when you’re going into B-cells. If you ...

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... may then be cloned into replicable cloning vectors using any method well known in the art. ... The present invention further includes an expression vector comprising phage operator and ... express the cloned polynucleotide. A typical mammalian expression vector contains a promoter ...

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