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Lysis

Definition

noun

(cytology)

The disintegration or rupture of the cell membrane, resulting in the release of cell contents or the subsequent death of the cell.

(medicine)

The gradual recovery or recuperation from an acute disease.

(suffix: -lysis)

Decomposition, breakdown or disintegration.


Supplement

(cytology) Lysis occurs as a result of viral or osmotic mechanisms that compromise the integrity of the cell membrane.


Word origin: from Greek λύσις, lysis from lyein = to separate. Related forms: lyse (verb), lytic (adjective).
Derived terms: cytolysis, dermatolysis, haemodialysis, haemolysis, enzymolysis, hydrolysis.


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Re: centrifugation of hemocytes

Are you resuspending in a PBS solution isotonic to the cells? Lysis could explain why the cells aren't there. You could try slightly increasing the tonicity of the PBS - better to have crenated cells than lysed cells.

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by jonmoulton
Mon Jan 27, 2014 10:44 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 2413

Weird - shaped band on western blotting

... band on my western blot. It is like an upside-down of smiling face in each lane. I use the same loading buffer, electrophoresis buffer, lysis buffer, and also the same 1st and 2nd antibody for this experiment. I also try to reduce the amount of protein in each lane to 25μg, and work ...

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by namakulaila
Thu Oct 17, 2013 6:17 am
 
Forum: Molecular Biology
Topic: Weird - shaped band on western blotting
Replies: 2
Views: 1315

Relationship between total protein and enzyme activity

Dear all, appreciate your comments here. I'm currently extracting GAD enzyme (Glutamate decarboxylase) by doing enzymatic lysis onto the fungus Aspergillus Oryzae.The lytic enzymatic cocktail was Yatalase, which composing of Chitinase, Chitobiase, Chitosanase, beta-1,3-glucanase and ...

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by schroddycat
Sun Sep 02, 2012 12:34 pm
 
Forum: Molecular Biology
Topic: Relationship between total protein and enzyme activity
Replies: 1
Views: 4729

Re: Amount of 2-mercaptoethanol in lysis buffer for western blot

My sample became sticky even after boiling for 10mins at 95degree and I wonder if this is because of the low volume of 2ME in the sample

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by yyua042
Thu Jul 12, 2012 9:40 pm
 
Forum: Molecular Biology
Topic: Amount of 2-mercaptoethanol in lysis buffer for western blot
Replies: 3
Views: 2336

Amount of 2-mercaptoethanol in lysis buffer for western blot

Hi all. I added 20uL of 2-mercaptoethanol in 600uL of lysis buffer (3% volume), and I used this to do my western and I got no signals on my membrane. I dont know if the reason why I got no signals is because that 3% of 2-mercaptoethanol was too low. ...

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by yyua042
Thu Jul 12, 2012 3:17 am
 
Forum: Molecular Biology
Topic: Amount of 2-mercaptoethanol in lysis buffer for western blot
Replies: 3
Views: 2336
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