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Lysis

Definition

noun

(cytology)

The disintegration or rupture of the cell membrane, resulting in the release of cell contents or the subsequent death of the cell.

(medicine)

The gradual recovery or recuperation from an acute disease.

(suffix: -lysis)

Decomposition, breakdown or disintegration.


Supplement

(cytology) Lysis occurs as a result of viral or osmotic mechanisms that compromise the integrity of the cell membrane.


Word origin: from Greek λύσις, lysis from lyein = to separate. Related forms: lyse (verb), lytic (adjective).
Derived terms: cytolysis, dermatolysis, haemodialysis, haemolysis, enzymolysis, hydrolysis.


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Relationship between total protein and enzyme activity

Dear all, appreciate your comments here. I'm currently extracting GAD enzyme (Glutamate decarboxylase) by doing enzymatic lysis onto the fungus Aspergillus Oryzae.The lytic enzymatic cocktail was Yatalase, which composing of Chitinase, Chitobiase, Chitosanase, beta-1,3-glucanase and ...

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by schroddycat
Sun Sep 02, 2012 12:34 pm
 
Forum: Molecular Biology
Topic: Relationship between total protein and enzyme activity
Replies: 1
Views: 2371

Re: Amount of 2-mercaptoethanol in lysis buffer for western blot

My sample became sticky even after boiling for 10mins at 95degree and I wonder if this is because of the low volume of 2ME in the sample

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by yyua042
Thu Jul 12, 2012 9:40 pm
 
Forum: Molecular Biology
Topic: Amount of 2-mercaptoethanol in lysis buffer for western blot
Replies: 3
Views: 985

Amount of 2-mercaptoethanol in lysis buffer for western blot

Hi all. I added 20uL of 2-mercaptoethanol in 600uL of lysis buffer (3% volume), and I used this to do my western and I got no signals on my membrane. I dont know if the reason why I got no signals is because that 3% of 2-mercaptoethanol was too low. ...

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by yyua042
Thu Jul 12, 2012 3:17 am
 
Forum: Molecular Biology
Topic: Amount of 2-mercaptoethanol in lysis buffer for western blot
Replies: 3
Views: 985

Re: how to make blood lysis buffer

I know i need to make stock solutions of the buffers but how do I make this into a solution together to make the appropriate concentrations of each chemical?

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by karenzellie
Fri May 25, 2012 2:48 pm
 
Forum: Molecular Biology
Topic: how to make blood lysis buffer
Replies: 3
Views: 847

how to make blood lysis buffer

Hi, I was wondering how I should make this recipe: 100mM Tris HCL 100mM EDTA 100mM Nacl 1%SDS

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by karenzellie
Thu May 24, 2012 5:12 pm
 
Forum: Molecular Biology
Topic: how to make blood lysis buffer
Replies: 3
Views: 847
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