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Dictionary » L » Lyse

Lyse

Definition

verb

To induce lysis, or to cause dissolution or destruction of a cell membrane with lysin.


Supplement

Word origin: from lysis < from Latin lysis, from Ancient Greek λύσις (“‘loosening’”).

Variants: lyze. Related forms: lysis (noun), lytic (adjective), lysed (simple past), lysing (present participle), lyses (simple present).


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Cell counting question

Tris is just a buffer http://en.wikipedia.org/wiki/Tris it does not lyse cells. It's basically an environment the cells are happy in because it has the right pH (your TA probably used Trist pH=7.4). The anomaly you witnessed was probably due to the fact that ...

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by MrMistery
Thu Aug 20, 2009 9:20 pm
 
Forum: Cell Biology
Topic: Cell counting question
Replies: 7
Views: 125

Cell counting question

... it to make it the correct volume. My question is this: What is tris, and what does it do. Would it have any effect on the cell population? Does it lyse cells? Could this have caused weird results with dividing cells? Thanks for any help!

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by jb0378
Thu Aug 20, 2009 5:11 pm
 
Forum: Cell Biology
Topic: Cell counting question
Replies: 7
Views: 125

Re: hypo and hyper osmotically??

... means that there are more solutes inside the cell (the salinity of the cell is higher than that of the outside). If the cell is animal, it will lyse, as water has the tendency to flow in areas of low solute concentration. If the cell is plant, it will become turgid (which is why plants stand ...

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by Chumbawamba
Fri Nov 21, 2008 4:14 am
 
Forum: Ecology
Topic: hypo and hyper osmotically??
Replies: 2
Views: 1238

Re: The Effect of pH on the Plant Cell Membrane

... residual blood cells--why do you think that might be important for correctly interpreting your results?). If the treatment causes the membranes to lyse, myoglobin is released and the solution turns pink. If you're lucky, the "amount" of coloration is proportional to the "amount" ...

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by blcr11
Wed Oct 08, 2008 1:00 pm
 
Forum: Molecular Biology
Topic: The Effect of pH on the Plant Cell Membrane
Replies: 6
Views: 2422

Re: Urea and membrane permeability of RBC

... urea) is isosmotic with plasma (as is 0.9% NaCl, or 5% glucose). A 0.28M solution of urea must be just hypotonic enough for water to flow into and lyse the RBCs. 1M urea should be hypertonic. The RBCs should shrivel up as water is driven out of the cells and they may not lyse at all. The issue ...

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by blcr11
Mon Aug 18, 2008 6:07 pm
 
Forum: Cell Biology
Topic: Urea and membrane permeability of RBC
Replies: 1
Views: 2133
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