Dictionary » L » Lysate



noun, plural: lysates

The solution consisting of the material released from cell lysis.


See also: lysis.

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use of a bacterial protease inhibitor cocktail with mammalia

... protease inhibitor cocktail) but it does not include the aprotinin or the leupeptin inhibitors. Is there a risk of proteolysis in a mammalian cell lysate without these latter 2 inhibitors? The specific cell types which we are using are: HUVEC (human umbilical vein endothelial cells) and HT-29 adenocarcinoma ...

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by rpwdoc
Tue Aug 26, 2014 10:08 pm
Forum: Cell Biology
Topic: use of a bacterial protease inhibitor cocktail with mammalia
Replies: 0
Views: 392

Simulated intracellular fluid

It is difficult to simulate intracellular fluid by using chemical reagent. Another way is using NP40 or OG to lysate the cell and collect the cell lysate. Then spin down the proteins and get the intracellular fluid. Will ___________________________________________________________________________________________ ...

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by agb06
Fri Sep 13, 2013 2:28 am
Forum: Cell Biology
Topic: Simulated intracellular fluid
Replies: 1
Views: 1187

In Vitro Transcription Translation

... gel with S-35 already added in reaction and autoradiographed to visulalize.. Do i have to also prove by western blot ? There are no background lysate proteins shown up in gel. Its pretty good. Any advice. I want to save time and money both as mine is not a funded project. I will appreciate ...

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by Shaanu
Mon Jun 03, 2013 7:12 am
Forum: Molecular Biology
Topic: In Vitro Transcription Translation
Replies: 3
Views: 2054

How can a protein without a G-site bind to GSH?

Hello can someone help me understand my situation? i am new at this. I used GSH-Sepharose 4B beads on a cell lysate and got a single protein band on SDS-PAGE. I sent it for MALDI-TOF, the sequence came back with 3% coverage that has homolog with acetyl carrier protein. question: ...

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by hurzz
Mon Nov 12, 2012 12:34 pm
Forum: Molecular Biology
Topic: How can a protein without a G-site bind to GSH?
Replies: 11
Views: 3567

Real Time reverse transcription PCR Questions

... (Real Time reverse transcription PCR). The protocol I have in mind is to transfect my cells with my desired proteins and when I split my cell lysate I will keep one for protein and one to isolate RNA. Then I will extract mRNA and use reverse transcription to make cDNA. I will be using the ...

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by wessH
Sun Jun 03, 2012 2:21 pm
Forum: Molecular Biology
Topic: Real Time reverse transcription PCR Questions
Replies: 0
Views: 2490
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