Dictionary » L » Lysate



noun, plural: lysates

The solution consisting of the material released from cell lysis.


See also: lysis.

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Simulated intracellular fluid

It is difficult to simulate intracellular fluid by using chemical reagent. Another way is using NP40 or OG to lysate the cell and collect the cell lysate. Then spin down the proteins and get the intracellular fluid. Will ___________________________________________________________________________________________ ...

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by agb06
Fri Sep 13, 2013 2:28 am
Forum: Cell Biology
Topic: Simulated intracellular fluid
Replies: 1
Views: 1073

In Vitro Transcription Translation

... gel with S-35 already added in reaction and autoradiographed to visulalize.. Do i have to also prove by western blot ? There are no background lysate proteins shown up in gel. Its pretty good. Any advice. I want to save time and money both as mine is not a funded project. I will appreciate ...

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by Shaanu
Mon Jun 03, 2013 7:12 am
Forum: Molecular Biology
Topic: In Vitro Transcription Translation
Replies: 3
Views: 1921

How can a protein without a G-site bind to GSH?

Hello can someone help me understand my situation? i am new at this. I used GSH-Sepharose 4B beads on a cell lysate and got a single protein band on SDS-PAGE. I sent it for MALDI-TOF, the sequence came back with 3% coverage that has homolog with acetyl carrier protein. question: ...

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by hurzz
Mon Nov 12, 2012 12:34 pm
Forum: Molecular Biology
Topic: How can a protein without a G-site bind to GSH?
Replies: 11
Views: 3305

Real Time reverse transcription PCR Questions

... (Real Time reverse transcription PCR). The protocol I have in mind is to transfect my cells with my desired proteins and when I split my cell lysate I will keep one for protein and one to isolate RNA. Then I will extract mRNA and use reverse transcription to make cDNA. I will be using the ...

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by wessH
Sun Jun 03, 2012 2:21 pm
Forum: Molecular Biology
Topic: Real Time reverse transcription PCR Questions
Replies: 0
Views: 2386

HCL instead of imidazole!

... a gel...I got a relatively faint band with the HCl elutent but have kept that separetly in the fridge for time being. After I sonicated, I ran my lysate thru the colum and i ran the FT on gel as well and theres quite a bit of protein there..so I dialyzed in MCAC-0 and tomorrow I will pass it through ...

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by biology_06er
Fri Dec 16, 2011 9:37 am
Forum: General Discussion
Topic: HCL instead of imidazole!
Replies: 6
Views: 2035
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