Lux

... light levels so I put them in a slightly shaded window with very gentle aeration. After two weeks I placed them on a light bench with around 5000 lux of standard white fluorescent light (this as the only value I could find on-line). I have since removed the aeration in case this was having an ...

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... the answer to my question. HOW DO YOU FIND THE LOCATION OF A GENE/OPERON? For my molecular genetics class, I have to find the location of the lux genes in V. fischeri. I've read a lot online that you can amplify DNA using PCR to get the sequence but that doesn't make sense to me. How can you ...

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Hi every one can you explain to me the lux genes please. I’ve read: "Note: Bacteria transformed with the pVIB plasmid must be grown at 30° C or less." does this apply to the protein responsible doesn’t function under 30c or the protein ...

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... to see it glow. On the other hand luciferase is chemiluminescence, it needs a substrate and create light. Usually plasmids includes a whole lux operon which will provide the pathway for the substrate synthesis and the luciferase to make it glow.

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I think LUX methods might work. Certainly, you should be able to make the labelled products, but whether you can quantitatively distinguish the two types of transcripts with LUX or not I’m not sure. If you run the pcr products ...

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