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Luciferase

Luciferase

(Science: enzyme) An enzyme from firefly tails that catalyses the production of light in the reaction between luciferin and atp. Used by the male firefly for producing light to attract females and used in the laboratory in a chemiluminescence bioassay for atp. An oxidising enzyme acting on luciferin, a substance that gives certain organisms an ability to emit light.


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Re: Science vs. Science Fiction, non-scientist needs help

... http://www.dbi.ca/books/PDFs/Water-Paper.PDF). [*]Bioluminescent bacteria often use molecules such as Luciferase (http://en.wikipedia.org/wiki/Luciferase). https://microbewiki.kenyon.edu/index.php/Bioluminescent_Bacteria_in_Human_Settings and http://cibt.bio.cornell.edu/workshops_and_summer_programs/0610ccc/Biolum.pdf ...

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by sosippus
Wed Sep 24, 2014 8:16 pm
 
Forum: Microbiology
Topic: Science vs. Science Fiction, non-scientist needs help
Replies: 2
Views: 781

Gene deletion

Use the same protocol, just modify it - substitute Luciferase or other fluorescent protein gene in place of an antibiotic resistance gene.

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by Cat
Sun Sep 15, 2013 3:47 pm
 
Forum: Molecular Biology
Topic: Gene deletion
Replies: 5
Views: 2429

Cell Based Assays vs. Animal Studies in Drug Discovery

... risks and liabilities of cell based reporter assays, including non-natural protein based reporter assays (e.g. circularly permuted firefly luciferase or GFP based reporter assays) and stem cell based assays, in drug discovery and development. Scientific arguments have been put forward to ...

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by TMJohnson
Mon Jan 16, 2012 6:16 am
 
Forum: Cell Biology
Topic: Cell Based Assays vs. Animal Studies in Drug Discovery
Replies: 0
Views: 978

cloning

Hello, I would like to insert an enhancer element into a cis-reporter plasmid (luciferase) to test promoter activity of my gene of insert. I found the consensus enhancer element sequence for my gene, consisting of only about 9 nucleotides. However, when I look ...

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by kk
Tue May 31, 2011 9:30 am
 
Forum: Molecular Biology
Topic: cloning
Replies: 10
Views: 5661

genetically modified, transgenic organisms; help.

... accomplished it would take years? I'm not trying to do something someone hasn't done, but something that i haven't done. I have been told that the luciferase is more difficult to work with, but the green flourescant protien is not exactly what i'm looking for. I don't even know where to begin with ...

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by lwpolkadot
Thu Oct 07, 2010 5:02 pm
 
Forum: Genetics
Topic: genetically modified, transgenic organisms; help.
Replies: 8
Views: 6475
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