Long-winded

... it with black paper. Keep the plates inverted under wooden box and on the light... and off that after half an hour .... try to maintain it for long as 5hours with a intermediate half an hour gap. I tried it in my lab as we had some Incubator problem during my research and it worked, even though ...

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... it's a risky method to control baby, but for your information, I don't think you can get pregnant as his sperms has been exposed to air for such a long time.

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... that dense in terms of colony numbers. The plates are only grown overnight at 37 degrees so I don't think its that they are being incubated too long. What might be the cause of this? The only thing I can think might be the problem is her antibiotic being degraded/the wrong concentration? (ampicillin) ...

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The purpose of dephosphorylation is to prevent self-ligation of empty vector. So unless your insert is very long, it should not be necessary.

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... phosphorylate or dephosphorylate any of the two? As far as I understand, it is not necessary to dephosphorylate my double-digested open vector as long as it was cut with two different enzymes. Thus, the 5'-end of my vector has a phosphate. But still, should I phosphorylate my insert, since its ...

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